胆固醇-g-聚乳酸对成纤维细胞生长和分化的影响  

Effect of Cholesteryl Oligo-graft-lactic Acid on Growth and Differentiation of Fibroblast

作　　者：王炜[1] 张瑞[1] 倪妮[1] 杨晔[1] 高向东[2] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]中国药科大学药学院,江苏南京211198

出　　处：《广州化工》2017年第4期57-59,共3页GuangZhou Chemical Industry

基　　金：国家中医药管理局国家中医临床研究基地业务建设科研专项课题;基于微血管修复的功能化皮肤组织工程支架设计与促糖尿病皮肤创伤愈合研究(JDZX2015128)

摘　　要：以开环聚合法制备一系列CLA低聚物;红外光谱法、偏光显微镜等方法鉴定产物结构与液晶态,评价液晶态CLA对小鼠胚胎成纤维细胞黏附和增殖的影响;以α-平滑肌肌动蛋白为指标,采用蛋白质印迹法评价CLA对成纤维细胞向肌成纤维细胞表型转化的影响。研究发现三种CLA低聚物在37℃下均处于液晶态,能够显著促进细胞黏附、增殖和α-平滑肌肌动蛋白表达。结果表明具有仿ECM结构的液晶态CLA低聚物能显著提高NIH-3T3的黏附和增殖,并对成纤维细胞表型转化具有促进作用。A series of CLAs were synthetised by ring- opening polymerization of lactide. Their structure and liquid crystalline state were identified by infrared spectroscopy and polarizing microscope. Their effects on 3T3 adhesion and proliferation were analysed by CCK- 8. Their effects on 3T3 differentiation were analysed by western blot using α-smooth muscle actin as the indication. The results indicated that CLAs with grafting ratios of 1∶1. 5,1∶2. 5 and 1∶3. 5were in liquid crystal phase at 37 ℃. CLA can improve 3T3 adhesion proliferation and the express of α- smooth muscle actin compared to control and PLA. It was found that CLAs can self- assemble into liquid crystal and promote improved3T3 growth and differentiation.

关 键 词：液晶 CLA 成纤维细胞 表型转化 

分 类 号：R641[医药卫生—外科学]