基于枸杞为原料的碳量子点制备及作为荧光探针高灵敏检测D-青霉胺  

Preparation of carbon quantum dots from lycium chinensis and as a fluorescent probe for high sensitive detection of D-penicillamine

作　　者：胡月芳[1,2] 张亮亮 林丽云 李雪凤 赵书林 梁宏 

机构地区：[1]省部共建药用资源化学与药用分子工程国家重点实验室广西师范大学化学与药学学院,桂林541004 [2]贺州学院化学与生物工程学院,贺州542899

出　　处：《中国科学：化学》2017年第2期258-266,共9页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21575031);广西自然科学基金(编号:2015GXNSFDA139006)资助项目

摘　　要：以枸杞为原料,一步水热法制备得到具有强烈荧光的碳量子点(CQDs),并基于Cu(Ⅱ)与CQDs形成无辐射复合体,构建了"关-开"型荧光探针用于D-青霉胺(D-PA)的选择性高灵敏检测.在CQDs溶液中加入Cu(Ⅱ)离子后,CQDs的荧光发生淬灭,体系的荧光信号处于"关闭"状态.在D-PA存在下,由于D-PA与Cu(Ⅱ)具有更强的结合力,形成比CQDs-Cu(Ⅱ)复合体更稳定的络合物,将Cu(Ⅱ)从CQDs表面移除下来,使CQDs的荧光得到恢复,体系的荧光信号呈"打开"状态.实验探讨了Cu(Ⅱ)对CQDs荧光淬灭的机理,考察了各种反应条件.在优化的条件下,CQDs探针的荧光恢复程度与D-PA的浓度在0.5~80μmol L^(-1)范围呈良好的线性关系,检出限为0.15μmol L^(-1).应用于人血清中D-PA的检测,回收率为96.7%~105.0%,相对标准偏差小于3.1%,表明该法可应用于人体内D-青霉胺药物的检测和药代动力学研究.A one-step hydrothermal method was developed for the preparation of carbon quantum dots（CQDs）with strong fluorescence by using lyciumchinensis as precursors,and a turn-on fluorescent probe based on Cu（Ⅱ）forming radiation free complex to CQDs was developed for detection of D-Penicillamine（D-PA） with high sensitivity and selectively.After Cu（Ⅱ） ions were added in CQDs solution,the fluorescence of CQDs was quenched,and the fluorescence signal of the system is in the ＂off＂ state.In the presence of D-PA,a more stable complex than the CQDs-Cu（Ⅱ） complex was formed due to D-PA and Cu（Ⅱ） have a stronger binding force,which result in the departure of Cu（Ⅱ） from the surface of the CQDs,and the fluorescence signal of the system was ＂open＂.The mechanism of fluorescence quenching of CQDs by Cu（Ⅱ） was studied,and the reaction conditions were optimized.Under optimal conditions,the fluorescence recovery degree of the CQDs probe showed a good linear relationship with the concentration of D-PA from 0.5 to 80 umol L^-1.The limit of detection was estimated to be 0.15 umol L^-1.The present method was successfully applied for the detection of D-PA in human serum.Recoveries were found to be in the range of96.7%-105%,and the result of statistical calculation shows that relative standard deviations（RSDs） were lower than3.1%.These results indicate that present method can be applied for the detection of D-AP in the human serum and pharmacokinetics investigation of D-AP.

关 键 词：枸杞 碳量子点 荧光探针 D-青霉胺的检测 

分 类 号：O657.3[理学—分析化学]