发光金属Salen配合物作为检测细胞内微黏度的荧光成像探针  

Luminescent metal salen complex as intracellular microviscosity fluorescent sensor

作　　者：尹昊琰 唐娟[1] 张俊龙[1] 

机构地区：[1]北京分子科学国家实验室(筹)稀土材料化学及应用国家重点实验室北京大学化学与分子工程学院,北京100871

出　　处：《中国科学：化学》2017年第2期267-276,共10页SCIENTIA SINICA Chimica

基　　金：国家重点基础研究发展计划(编号:2015CB856301);国家自然科学基金(编号:21271013;21571007)资助项目

摘　　要：细胞内微黏度是描述细胞状态的重要物理参数,与物质转运和信号转导等一系列扩散控制的生理过程密切相关.构筑对细胞内微黏度响应灵敏的荧光探针是原位实时检测细胞状态的重要手段之一.我们设计含有D-π-A推拉电子结构N,N'-双水杨醛缩乙二胺类配体(N,N'-thiophene-3,4-bis-4'-(diethylamino)-salicylimine,Salen)的Zn^(2+)和Al^(3+)配合物,考察了其光物理性质,发现该类配合物具有对溶液黏度依赖的荧光增强性质.同时,相比于配合物ZnSalen,[AlSalen]^+Cl^-具有检测灵敏度高、荧光成像信号增强倍数高等优点.利用激光扫描共聚焦荧光成像和荧光寿命成像,[AlSalen]^+Cl^-同样表现出在培养温度降低或细胞自噬下的荧光增强和发光寿命延长等变化,显示其作为检测细胞内微黏度荧光探针的潜在应用价值.Microviscosity in living cells influences the diffusion of biomolecules and is an important physical parameter that related to cellular pathological processes.Although several fluorescent molecular rotors have been reported to work as intracellular microviscosity sensors,luminescent metal complexes have much less been studied.Herein we used zinc and aluminum complexes of Salen ligand（N,N＇-thiophene-3,4-bis-4＇-（diethylamino）-salicylimine） as fluorescent sensors for monitoring intracellular microviscosity.Although both of them showed enhanced fluorescence quantum yield along with increasing solvent viscosity,[AlSalen]^＋Cl^- displayed higher sensitivity and stronger intracellular fluorescence signal,making it a better choice for intracellular application as microvisicosity sensor.Moreover,the fluorescence lifetime of[AlSalen]^＋Cl^- also increased in higher viscosity environment,which has been demonstrated in monitoring microviscosity raising during incubating temperature decrease or autophagy process of HeLa cell via fluorescence lifetime imaging microscopy（FLIM）.

关 键 词：发光金属配合物 荧光探针 细胞内微黏度 细胞成像 荧光寿命成像 

分 类 号：O641.4[理学—物理化学]