miR-205调控YES1表达对人膀胱癌细胞增殖的影响  

The effect of miR-205 on the proliferation of human bladder cancer cells by directly repressing YES1

作　　者：苏允伟 祝兴旺[1] 杨玉彬[1] 刘锁民[1] 薛东炜[1] 刘屹立[1] 

机构地区：[1]中国医科大学附属第四医院泌尿外科,辽宁沈阳110032

出　　处：《解剖科学进展》2017年第1期54-56,60,共4页Progress of Anatomical Sciences

基　　金：辽宁省科学计划项目(2015225009)

摘　　要：目的探讨miR-205对膀胱癌T24细胞增殖的影响。方法合成miR-205的模拟物并转染膀胱癌T24细胞,荧光显微镜下观察转染效率,实时荧光定量PCR法检测转染48 h后,T24细胞中miR-205的表达情况,MTT法检测转染24、48和72 h后,T24细胞的增殖情况,实时荧光定量PCR法和western blot检测转染48 h后膀胱癌细胞内源性YES1 mRNA及蛋白的表达变化。结果 miR-205的模拟物转染至T24细胞的效率为80%,转染miR-205模拟物48 h后,T24细胞内miR-205的表达水平显著升高(P<0.05),T24细胞的增殖率显著低于阴性对照组(P<0.05),且呈时间依赖性,YES1 mRNA及蛋白的表达水平明显低于阴性对照组(P<0.05)。结论 miR-205抑制膀胱癌T24细胞增殖可能与靶向YES1基因相关。Objective To investigate the effect of miR-205 on the proliferation of human bladder cancer cells by directly repressing YES1. Methods miR-205 mimics and miR-205 mimics negative control were synthesized and transfected into bladder cancer T24 cells, the transfection efficiency was observed under a fluorescence microscope. The expression level of miR-205 in T24 cells w.as detected by real-time PCR at 48 h after transfection, the proliferation of T24 cells was detected by MTT assay. The expression of YES1 mRNA and protein in T24 cells was detected by real-time PCR and Western blot methods respectively after miR-205 mimics was transfected. Results The efficiency of miR-205 mimics transfection into T24 cells was 80%. Compared with negative control group, the expression level of miR-205 in T24 cells was higher after transfection with miR-205 mimics （ P〈0.05 ） , the proliferative rates of T24 cells were lower after transfeetion with miR-205 mimics （P〈0.05） with a timedependent manner. The expression levels of YES1 mRNA and protein in T24 cells were lower in transfection miR-205 mimics group than in the negative control group （P〈0.05） . Conclusion The inhibition of MiR-205 on the proliferation of T24 cells might be related to repressing YES1 gene.

关 键 词：miR-205 膀胱癌 YES1 增殖 T24细胞 

分 类 号：Q737.14[生物学—分子生物学]