Tamoxifen诱导敲除多囊肾小鼠Pkd1基因后的肾脏病理变化  

Pathological Change of Kidney in Pkdl Knock-out Mice with Polycystic Kidney Induced by Tamoxifen

作　　者：周卫民[1] 朱科燕[1] 陈方明[1] 蔡月琴[1] 方明笋[1] 齐月寒 陈民利[1] 

机构地区：[1]浙江中医药大学动物实验研究中心/比较医学研究中心,杭州310053

出　　处：《实验动物与比较医学》2017年第1期11-14,共4页Laboratory Animal and Comparative Medicine

基　　金：浙江省公益性技术应用研究计划项目(2014C37009);浙江中医药大学比较医学创新团队(XTD201301)

摘　　要：目的探讨Tamoxifen诱导下敲除Pkd1^(f/f):Cre转基因小鼠多囊肾病1(Pkd1)基因前后的肾功能变化情况。方法选用出生11 d的Pkd1^(f/f):Cre转基因小鼠和Pkd1f/f野生型小鼠用Tamoxifen进行诱导,分别于诱导后7d、14d、21d检测血清肌酐与尿素氮含量,检测21 d时体质量、肾脏重量及其脏器系数。结果诱导敲除后7d小鼠血清肌酐与尿素氮含量开始升高,14d、21 d小鼠血清肌酐与尿素氮含量明显升高(P<0.01)。21 d时诱导敲除小鼠肾脏系数明显高于未敲除小鼠。结论 Tamoxifen能使条件诱导敲除pkd1基因的小鼠肾功能产生明显改变,肾组织出现大量囊泡症状。Objective To explore the change of kidney function of polycystic kidney disease （PKD） mice before and after the knockout Pkdlf/f： Cre gene induced by Tamoxifen induction. Methods The Pkdlf/t：Cre transgenic mice, Pkdlf/f：Cre wild type mice aged on 11 days were induced by Tamoxifen, the contents of serum creatinine and urea nitrogen were detected at 7 days, 14 days and 21days after induction. The body weight, kidney weight and the kidney viscera coefficient were detected after 21 day. Result After 7 days induction, the contents of serum creatinine and urea nitrogen began to rise after 14 days and 21 days induction, the parameters were significantly increased （P〈0.01） After 21 days induction, the kidney coefficient of knocked out transgenic mice kidney coefficient was higher than that of wild type mice. Conclusion The renal function of Pkdlf/f：Cre transgenic mice were obviously changed by Tamoxifen induction, the kidney of transgenic mice showed symptoms of a large number of vesicles.

关 键 词：多囊肾(PKD) 转基因小鼠 肾功能 TAMOXIFEN 诱导 

分 类 号：R-332[医药卫生] R692