榆耳酸溶性多糖对肝癌H_(22)小鼠的抑瘤作用研究  

A Research on the Anti-tumor Effects of Acid-Soluble Polysaccharides from Gloeostereum incamatum in H_(22) Tumor Bearing Mice

作　　者：王红[1] 赵岩[1] 蔡恩博[1] 图力古尔[2] 李伟[1] 李玉[2] 包海鹰[1,2] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林农业大学食药用菌教育部工程研究中心,长春130118

出　　处：《世界科学技术-中医药现代化》2017年第1期142-148,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：教育部长江学者与创新团队项目(IRT_15R25):重要菌物资源保育与可持续利用研究;负责人:图力古尔

摘　　要：目的:研究榆耳酸溶性多糖对小鼠H22移植瘤的生长抑制作用及其机理。方法 :以抑瘤率、肝指数、胸和脾脏指数、白细胞介素-2、γ-干扰素、肿瘤坏死因子-α等指标来考察榆耳酸溶性多糖对H22荷瘤小鼠肿瘤抑制作用,以H&E染色法观察肿瘤组织的病理改变,采用TUNEL法检测细胞凋亡情况。结果:榆耳酸溶性多糖3个剂量组能显著抑制肿瘤的增殖,提高荷瘤小鼠脾脏、胸腺指数以及血清细胞因子IL-2、IFN-γ和TNF-α;肿瘤组织H&E染色和TUNEL实验表明,榆耳酸溶性多糖可以通过诱导肿瘤细胞凋亡而发挥抑制肿瘤生长作用。结论:榆耳酸溶性多糖具有显著的抗肿瘤作用,其作用机制可能与免疫调节、促细胞凋亡、保护肝脏功能有关。This study aimed at exploring the inhibitory effect behind its mechanism on acid-soluble polysaccharides from G. incamatum in transplanted H_（22） tumor mice. Different indices, including tumor inhibitory rate, organ index of liver, thymus and spleen, IL-2, IFN-γ and TNF-α were detected for the evaluation of anti-tumor effects and the mechanism. Furthermore, HE staining and TUNEL assay were adopted to investigate the pathological changes of tumor tissue and cell apoptosis, respectively. As a result, the three dose groups of acid-soluble polysaccharides of G. incamatum successfully inhibited the proliferation of tumor cells, while organ indexes of spleen and thymus were improved and serum IL-2, IFN-γ and TNF-α increased. HE staining and TUNEL assay showed the polysaccharides induced cell apoptosis, playing a significant role in the inhibition of tumor growth. In conclusion, acid-soluble polysaccharides of G. incamatum possessed significant anti-tumor effects, behind which the mechanism could be related to the regulation of immune regulation, cell apoptosis, and the protection of liver function.

关 键 词：榆耳酸溶性多糖 H22荷瘤小鼠 抗肿瘤 H＆E染色 TUNEL 

分 类 号：R285.5[医药卫生—中药学]