洋金花总碱对人肺腺癌A549细胞EMT过程中E-cad表达的影响  

Effect of Yangjinhua Alkaloids on the Expression of E-cad in Human Lung Adenocarcinoma Cell Line A549 Cells during EMT

作　　者：谭晓丽[1] 吕晓东[1] 王真[2] 

机构地区：[1]浙江省嘉兴市第一医院呼吸科,嘉兴314000 [2]浙江省中医院呼吸科,杭州310006

出　　处：《浙江中西医结合杂志》2017年第2期89-92,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2012ZB049)

摘　　要：目的观察洋金花总碱对转化生长因子-β1(TGF-β1)诱导的A549细胞向间质细胞转化(EMT)过程中E-钙黏蛋白(E-cad)表达的影响。方法将体外培养的A549细胞随机分为空白对照组,TGF-β1组及洋金花总碱低、中、高浓度药物组,分别作用24、48、72h后,通过Western-blot及RT-PCT检测各组A549细胞E-cad表达的变化。结果 24、48、72h E-cad表达,空白对照组分别为(1.49±0.12)、(2.52±0.07)、(2.58±0.21);TGF-β1组分别为(0.66±0.01)、(0.50±0.13)、(0.52±0.08);洋金花总碱低、中、高浓度药物组分别为24h[(0.67±0.12)、(0.67±0.12)、(1.12±0.05)]、48h[(0.70±0.28)、(1.02±0.08)、(1.10±0.13)]、72h[(0.68±0.04)、(1.25±0.11)、(1.30±0.09)]。与空白对照组比较,TGF-β1组和洋金花总碱低、中、高浓度药物组的E-cad表达均明显减少(P<0.01);与TGF-β1组比较,各时间段的洋金花总碱低浓度组E-cad表达无明显变化(P>0.05),但48、72h洋金花总碱中、高浓度组E-cad表达明显增加(P<0.01);与24h低浓度药物组比较,24h中浓度药物组Ecad表达差异无统计学意义(P>0.05),但作用48、72h后,中浓度药物组的E-cad表达明显增加(P<0.01),而高浓度药物组3个时间点的E-cad表达均明显增加(P<0.01)。与中浓度药物组比较,24h高浓度药物组E-cad表达增加(P<0.05),48、72h高浓度药物组E-cad表达差异无统计学意义(P>0.05)。与空白对照组比较,不同浓度作用的洋金花总碱药物组使E-cad m RNA表达上调(P<0.01),并且在72h及高浓度时最明显(P<0.01)。结论洋金花总碱通过增加上皮标志物E-cad表达,可抑制A549细胞的上皮间质转化。Objective To observe the E-cad expression changes in A549 cells during TGF-β1-induced EMT after Yangjinhua alkaloids treatment. Methods A549 cells in vitro cultured were randomly divided into blank control group, TGF-β1 group, low, medium and high dose of Yangjinhua alkaloids group. The change of cell morphology and the expression of E-cad were observed by western blot and RT-PCR after 24, 48 and 72 hours, respectively. Results The expressions of E-cad at 24, 48 and 72 hours after treatment were 1.49 ±0.12, 2.52 ±0.07,2.58±0.21 in blank control group, 0.66±0.01, 0.50±0.13, 0.52±0.08 in TGF-β1 group, 0.67±0.12, 0.67±0.12, 1.12±0.05 in low dose of Yangjinhua alkaloids group, 0.70±0.28, 1.02±0.08, 1.10±.13 in medium dose of Yangjinhua alkaloids group and 0.68 ±0.04, 1.25 ±0.11, 1.30 ±0.09 in high dose of Yangjinhua alkaloids group. Compared with those in blank control group, the expressions of E-cad in three Yangjinhua alkaloids groups were significantly decreased（P〈0.01）. Compared to those in TGF-β1 group, the expressions of E-cad at 24, 48 and 72 hours in low dose of Yangjinhua alkaloids group were not different（P〈0.05）, but the expressions of E-cad at 48 and 72 hours in medium and high doses of Yangjinhua alkaloids groups were obviously increased（P 0.01）. Among three Yangjinhua alkaloids groups, the expression of E-cad at 24 h in low dose group did not differ from that in medium dose group, but the expression of E-cad at 48 and 72 hours in medium dose groups were significantly increased（P〈0.01）, and the expression of E-cad in high dose group were higher at each time point（P〈0.01）; compared to those in medium dose group, the expression of E-cad at 24 h in high dose group were raised（P〈0.05）,but no significant difference was noted between two groups at 48 and 72 hours（P〈0.05）. Compared to blank control group, three Yangjinhua alkaloids groups all had higher level of E-cad m RNA（P〈0.01）, and the level of Ecad m RNA reached the peak at 72 hours after tr

关 键 词：肺腺癌细胞 A549细胞 洋金花总碱 上皮细胞间质化 E-CAD 

分 类 号：R285[医药卫生—中药学]