苹果砧木组培苗生根诱导技术研究  

Rooting Induction Technique of Apple Stock Tissue Culture Plantlets

作　　者：郭成[1] 葛红娟[2] 赵玲玲[3] 王成荣 束怀瑞[1] 沙广利[2] 张世忠[1] 

机构地区：[1]山东农业大学生命科学学院/作物生物学国家重点实验室,山东泰安271018 [2]青岛市农业科学研究院,山东青岛266100 [3]烟台市农业科学研究院,山东烟台265500 [4]章丘市相公中学,山东章丘250203

出　　处：《山东农业科学》2017年第2期72-75,共4页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31401822);中国博士后科学基金面上资助项目(2014M561952)

摘　　要：以平邑甜茶无根试管苗为材料,以1/2MS培养基、育苗基质、黏土及沙子作为培养介质,研究NAA浓度及培养介质对平邑甜茶试管苗生根的影响。结果表明:育苗基质作为培养介质进行微枝扦插的试管外生根技术效果最好,成活率最高,达到94%,根系活力达240.82μg/(g·h)。Using the rootless test-tube plantlets of Malus hupenensis Rhed. as experimental materials,the effects of NAA concentration and media on rooting were researched with 1 /2MS medium,plug seedling substrate,clay and sand as media. The results showed that using plug seedling substrate as the medium for ex vitro rooting of micro-softwood cutting had the best effects with the highest survival rate of 94% and the root activity of 240. 82 μg /（ g·h）.

关 键 词：平邑甜茶 微枝扦插 无根驯化 试管外生根 

分 类 号：S661.104.3[农业科学—果树学]