马钱子配伍苏木对关节炎大鼠抗炎及免疫机制的影响  

Effect of Anti-Inflammatory and Immunologic Mechanism on Rats with Arthritis Treated with Maqianzi and Sumu

作　　者：梁晓东[1,2] 边月娟 李茜[2] 唐迎雪[2] 

机构地区：[1]中国中医科学院西苑医院老年病研究所,北京100091 [2]山东中医药大学中医学院,山东济南250355 [3]American Academy of Acupuncture and Oriental Medicine,Roseville MN 55113,American

出　　处：《山东中医杂志》2017年第2期151-154,共4页Shandong Journal of Traditional Chinese Medicine

基　　金：高等学校博士学科点专项科研基金(编号:200804410002)

摘　　要：目的:探讨马钱子配伍苏木对佐剂性关节炎(AA)大鼠的免疫分子机制及较佳配比。方法 :马钱子与苏木不同配比水煎剂连续灌胃给药32 d,取血和滑膜组织,观察滑膜组织病理变化,采用酶联免疫吸附法(Elisa)测定血清中白介素-1β、白介素-6、白介素-10(IL-1β、IL-6、IL-10)、循环免疫复合物(CIC)、肿瘤坏死因子(TNF-α)、C型反应蛋白(CRP);通过逆转录-聚合酶链反应(RT-PCR)的方法,检测AA大鼠滑膜IL-1β、IL-10、TNF-αm RNA的表达情况。结果:不同配比的马钱子与苏木煎剂,能改善AA大鼠的滑膜充血、炎细胞浸润等病理变化,不同程度降低炎性细胞因子IL-1β、TNF-α、IL-6、CIC、CRP的含量;降低IL-1βm RNA的表达,上调IL-10 m RNA的表达。结论 :马钱子与苏木配伍对AA大鼠具有一定抗炎作用,其机制为抑制炎性细胞因子,控制炎症反应,其较佳配比为1∶6。Objective：To discuss the anti-inflammatory and immunologic mechanism on rats with adjuvant arthritis（AA） treated with Maqianzi and Sumu,and to determine the optimized ratio of Maqianzi to Sumu.Methods：AA model rats were given decoction of Maqianzi and Sumu with different ratio through gavage for32 days. Synovial tissue was under pathological examination. Changes of interleukin-1β,-6,10（IL-1β,IL-6,IL-10）,tumor necrosis factor-α（TNF-α）,circulating immune complex（CIC）,C reactive protein（CRP） in serum were tested through ELISA,and m RNA expression of IL-1β,IL-10,TNF-α were detected through polymerase chain reaction（PCR）. Results：Maqianzi and Sumu combined together in different ratio could obviously reduce the content of IL-1β,IL-6,TNF-α,CIC,CRP,decrease the expression level of IL-1β and increase IL-10. Conclusion：Maqianzi and Sumu in combination had certain anti-inflammatory function,through suppressing inflammatory cytokines,and the optimized ratio was 1 to 6.

关 键 词：马钱子 苏木 配伍 细胞因子 免疫机制 

分 类 号：R285.5[医药卫生—中药学]