薏苡仁蛋白的聚丙烯酰胺凝胶电泳分析  

SDS-PAGE Analysis for Proteins in Coix Seed

作　　者：崔雪莹[1] 相美容 武皓[1] 容蓉[1] 蒋海强[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《山东中医杂志》2017年第2期155-157,共3页Shandong Journal of Traditional Chinese Medicine

基　　金：科技部国家重点基础研究发展计划973计划课题(编号:2013CB5318000);山东中医药大学创新训练项目(编号:2015106)

摘　　要：目的:比较酸沉前后蛋白种类的差异,获取薏苡仁蛋白分子量的信息。方法:采用碱溶酸沉法制备薏苡仁蛋白,SDS-PAGE电泳分离蛋白,标准曲线法计算分子量信息。结果:得到了6种薏苡仁水溶性蛋白条带,确定了其分子量分别是91.598,77.169,39.839,28.977,23.822,16.100 k Da,对比了薏苡仁蛋白酸沉前后的变化,即酸沉后缺失了23.822 k Da和16.1 k Da两个条带。结论:该方法得到的谱图清晰,重现性好,对薏苡仁蛋白分离与分析的研究有着重要的指导意义。Objective：To compare the differences of protein categories before and after acid-isolation and to study the protein molecular weights in Coix seed. Methods：An alkali-solution and acid-isolation method was employed to extract the proteins in Coix seed,and the protein was isolated by SDS-PAGE analysis,and the molecular weights were calculated by the standard curve method. Results：There were 6 bands of soluble protein,and the molecular weights were determined as follows ：91.598,77.169,39.839,28.977,23.822 and1 6.1 k Da. Proteins in Coix seed before and after acid-isolation were compared and it was found that two protein bands,23.822 k Da and 16.1 k Da,were missing. Conclusion：Through this method,the resulted spectrogram was clear with good repeatability,and could guide the isolation and analysis of proteins in Coix seed.

关 键 词：薏苡仁蛋白 十二烷基磺酸钠-聚丙烯酰胺凝胶电泳 分子量 

分 类 号：R284.2[医药卫生—中药学]