大黄素和DMSO对肺缺血-再灌注损伤的保护作用及其机制  

Protective effect and underlying mechanism of emodin and dimethyl sulfoxide on lung ischemiareperfusion injury

作　　者：李桂荣[1] 李慧星[1] 高霏[1] 杨振坤[1] 卫栋[1] 陈静瑜[1] 

机构地区：[1]南京医科大学附属无锡人民医院江苏省人体器官移植重点实验室,江苏省无锡市214023

出　　处：《江苏医药》2017年第3期153-156,F0002,共5页Jiangsu Medical Journal

基　　金：无锡市医管中心重大项目(YGZXZ1405)

摘　　要：目的探讨大黄素和DMSO对肺缺血-再灌注损伤的保护作用及其可能机制。方法24只SD大鼠随机均分为四组:A、B和C组大鼠在建立缺血-再灌注损伤模型前2h分别给予生理盐水5ml/kg、大黄素溶液5mg/kg和5%DMSO 5ml/kg,D组仅在术前2h给予生理盐水作为假手术组,开胸,心脏取血。采用HE染色观察肺组织病理学变化,计算肺组织的湿/干重比(W/DR),RT-PCR法检测线粒体DNA(mtDNA)的表达,ELISA法检测血清IL-6、IL-8和TNF-α的含量。结果与D组相比,A组肺泡中大量炎症物质渗出,肺间质水肿,肺组织W/DR增大,mtDNA表达以及IL-6、TNF-α、IL-8含量增加(P<0.05);而B组和C组分别给予大黄素和DMSO后,能有效改善上述各指标的变化过程(P<0.05)。结论大黄素和DMSO通过抑制mtDNA的释放,降低炎症因子及趋化因子的表达,减轻机体的炎症反应,从而对肺缺血-再灌注损伤起保护作用。Objective To investigate the protective effect and underlying mechanism of emodin and dimethyl sulfoxide（DMSO）on lung ischemia-reperfusion（I-R）injury.Methods Twenty-four SD rats were equally randomized into four groups.At 2hours before establishing the models with lung I-R injury,the rats in groups of A,B and C were treated with normal saline,emodin 5mg/kg and 5%DMSO 5 ml/kg,respectively.Group D was given normal saline only as sham operated.The pathological changes of lung tissues were observed by HE staining,and the ratio of wet to dry weight（W/DR）was calculated.The expression of mitochondrial DNA（mtDNA）was detected by RT-PCR.The contents of IL-6,IL-8and TNF-αwere determined by ELISA method.Results Compared with group D,a large number of inflammatory substances in the alveolus was exuded,lung interstitial edema appeared,W/DR ratio of lung tissues was enlarged,mtDNA expression and contents of IL-6,IL-8and TNF-αwere increased in group A（P〈0.05）,which were all significantly improved after treated with emodin and DMSO in groups of B and C,respectively（P〈0.05）.Conclusion Emodin and DMSO can protect the lungs from I-R injury probably via inhibiting the release of mtDNA,decreasing the expressions of inflammatory factors and chemokines and alleviating the inflammatory response of body.

关 键 词：大黄素 二甲基亚砜 线粒体DNA 肺缺血-再灌注损伤 

分 类 号：R392[医药卫生—免疫学]