薏仁对糖尿病肾病大鼠β1整合素及黏着斑激酶表达的影响  

Effect of coix seed on expressions of β1-integrin and focal adhesion kinase in rats with diabetic nephropathy

作　　者：蒋曦媛[1] 陆玉凤[1] 刘丽燕[1] 余江毅[2] 

机构地区：[1]昆山市中医医院内分泌科,江苏省215300 [2]江苏省中医院内分泌科

出　　处：《江苏医药》2017年第3期157-160,F0002,共5页Jiangsu Medical Journal

基　　金：昆山市社会发展科技计划项目(ks0910)

摘　　要：目的探讨薏仁对糖尿病肾病大鼠肾组织中β1整合素及黏着斑激酶(FAK)表达的影响。方法 50只大鼠随机均分为正常对照组(A组)、糖尿病肾病模型组(B组)、薏仁治疗组(C组)、厄贝沙坦治疗组(D组)和薏仁+厄贝沙坦联合治疗组(E组),C、D、E组分别以薏仁400mg·kg^(-1)·d^(-1)、厄贝沙坦20mg·kg^(-1)·d^(-1)、薏仁400mg·kg^(-1)·d^(-1)+厄贝沙坦20mg·kg^(-1)·d^(-1)水剂灌胃治疗至8周末。采用HE染色观察大鼠肾组织病理变化,qRT-PCR和Western blot分别检测β1整合素、FAK的mRNA及其蛋白表达。结果B组β1整合素及FAK的mRNA和蛋白表达均较A组增加(P<0.05);与B组相比,C、D、E组β1整合素及FAK的mRNA和蛋白表达降低(P<0.05);与C组相比,E组β1整合素及FAK的mRNA和蛋白表达略降低(P>0.05)。结论薏仁通过下调肾组织中β1整合素及FAK的表达,从而达到减轻肾小球纤维化、延缓糖尿病肾病进展的作用。Objective To investigate the effect of coix seed on the expressions ofβ1-integrin and focal adhesion kinase（FAK）in the renal tissues of rats with diabetic nephropathy（DN）.Methods Fifty rats were equally randomized into five groups of A（normal controls）,B（DN model）,C（treated with coix seed 400mg·kg^-1·d^-1）,D（treated with irbesartan 20mg·kg^-1·d^-1）and E（treated with coix seed 400mg·kg^-1·d^-1 plus irbesartan 20mg·kg^-1·d^-1）.The pathological changes of renal tissues were observed by HE staining.The mRNA and protein expressions ofβ1-integrin and FAK were detected by qRT-PCR and Western blot,respectively.Results The mRNA and protein expressions ofβ1-integrin and FAK in group B were higher than those in group A（P〈0.05）.Compared with group B,the mRNA and protein expressions ofβ1-integrin and FAK were decreased in groups of C,D and E（P〈0.05）.Moreover,compared with group C,the mRNA and protein expressions ofβ1-integrin and FAK were slightly decreased in group E（P〉0.05）.Conclusion Coix seed can protect renal function and delay DN progress probably via downregulating the expressions ofβ1-integrin and FAK in the renal tissues.

关 键 词：薏仁 糖尿病肾病 Β1整合素 黏着斑激酶 

分 类 号：R587[医药卫生—内分泌]