HPLC法测定参茸安神片中远志酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B和五味子醇甲  

Determination of polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, tenuifolin, onjisaponin B, and schisandrin in Shenrong Anshen Tablets by HPLC

作　　者：丁广军[1] 刘源[1] 陈慧慧[1] 

机构地区：[1]商丘市第一人民医院药学部,河南商丘476100

出　　处：《现代药物与临床》2017年第2期188-191,共4页Drugs & Clinic

摘　　要：目的建立HPLC法同时测定参茸安神片中远志酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B和五味子醇甲的方法。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈–0.1%磷酸溶液,梯度洗脱;检测波长:320 nm(0~16 min检测远志酮Ⅲ、3,6'-二芥子酰基蔗糖)、210 nm(16~24 min检测细叶远志皂苷、远志皂苷B)、250 nm(24~35 min检测五味子醇甲);体积流量:1.0 m L/min;柱温:30℃;进样量为10μL。结果远志酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B和五味子醇甲分别在3.85~77.00、6.22~124.40、6.57~131.40、7.61~152.20、4.96~99.20μg/m L线性关系良好(r≥0.999 2);平均回收率分别为98.17%、99.24%、99.90%、97.63%、96.83%,RSD值分别为1.14%、1.23%、0.70%、1.52%、0.81%。结论本法稳定可靠、简便易行,为全面控制参茸安神片的质量提供了参考。Objective To establish an HPLC method for simultaneous determination of polygalaxanthone Ⅲ, 3,6＇-disinapoyl sucrose, tenuifolin, onjisaponin B, and schisandrin in Shenrong Anshen Tablets. Methods The determination was carried out with Kromasil C18 column（250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile- 0.1% phosphoric acid solution with gradient elution. The detection wavelengths were 320 nm in 0 — 16 min（determination of polygalaxanthone Ⅲ and 3,6＇-disinapoyl sucrose）, 210 nm in 16 — 24 min（determination of tenuifolin and onjisaponin B）, and 250 nm in 24 — 35 min（ determination of schisandrin）. The flow rate was 1.0 m L/min, and the column temperature was set at 30 ℃ with injection volume of 10 μL. Results There were good linear relationships of polygalaxanthone Ⅲ, 3,6＇-disinapoyl sucrose, tenuifolin, onjisaponin B, and schisandrin in the concentration ranges of 3.85 — 77.00, 6.22 — 124.40, 6.57 — 131.40, 7.61 — 152.20, 4.96 — 99.20 μg/m L（r≥0.999 2）. The average recoveries were 98.17%, 99.24%, 99.90%, 97.63%, and 96.83% with RSD 1.14%, 1.23%, 0.70%, 1.52%, and 0.81%, respectively. Conclusion The method is simple and valid, which can be used in quantity control for Shenrong Anshen Tablets.

关 键 词：参茸安神片 远志[口山]酮Ⅲ 3 6＇-二芥子酰基蔗糖 细叶远志皂苷 远志皂苷B 五味子醇甲 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]