健脾疏肝颗粒对链脲佐菌素所致糖尿病小鼠的抗糖尿病效应及其作用机制的研究  

Therapeutic effects and underlying mechanism of Jianpishugan Particles in Streptozotocin-induced diabetic mice

作　　者：王素美[1] 曹永仓[1] 赵娟[1] 刘蓉[1] 

机构地区：[1]山东省泰安市中医医院,山东泰安271000

出　　处：《中国医院药学杂志》2017年第4期348-351,共4页Chinese Journal of Hospital Pharmacy

基　　金：山东省医药卫生科技发展计划(编号:2013BJYB36);山东省泰安市科技局立项科研(编号:20123073)

摘　　要：目的:探讨健脾疏肝颗粒对链脲佐菌素(STZ)糖尿病模型小鼠的药理作用和作用机制。方法:小鼠分为空白对照组(CNTL)、空白干预组(JPSG)、模型对照组(STZ)、药物低剂量组、高剂量组(STZ+JPSGD、STZ+JPSGG)各6,6,12,12,12只,健脾疏肝颗粒或纯化水灌胃20 d,检测小鼠空腹血糖(FBG)、胰岛素(FINS)、血脂、胰高血糖素、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px),计算胰岛素抵抗指数(HOMA-IR),以及胰高血糖素样肽-1(GLP-1)表达情况。结果:与模型对照组比较,STZ+JPSGD组、STZ+JPSGG组小鼠FBG、FINS、HOMA-IR、血脂、胰高血糖素均显著趋于正常,SOD、MDA、GSH-Px活性明显改善,GLP-1表达接近空白对照组,差异具有统计学意义(P<0.05或P<0.01)。结论:健脾疏肝颗粒对STZ模型小鼠具有明显降糖、降脂作用,作用机制可能通过抗氧化作用、调节GLP-1表达和FINS、胰高血糖素水平来发挥作用,值得进一步研究。OBJECTIVE To investigate the pharmacological effects and underlying mechanism of Jianpishugan Particles in streptozotocin（ STZ） induced diabetic mice. METHODS The mice were divided into blank control group（ CNTL）,blank intervention group（ JPSG）,model control group（ STZ）,low-dose drug group（ STZ ＋ JPSGD）,high-dose drug group（ STZ ＋ JPSGG） including6,6,12,12 and 12 animals,respectively. Jianpishugan Particles or distilled water were gavaged for 20 days. Blood glucose（ FBG）,insulin（ FINS）,blood lipids,glucagon,superoxide dismutase（ SOD）,malondialdehyde（ MDA）,glutathione peroxidase（ GSH-Px）were determined,insulin resistance index（ HOMA-IR） was calculated,pancreatic morphology and glucagon-like peptide-1（ GLP-1）expression were observed. RESULTS Compared with model control group,FBG,FINS,HOMA-IR,lipids,glucagon in STZ ＋ JPSGD group and STZ ＋ JPSGG group were significantly normalized,SOD,MDA and GSH-Px activities were obviously improved（ P 0.05 or P 0. 01）. GLP-1 expression of the two groups was close to that of the control group. CONCLUSION The significantly hypoglycemic,lipid-lowering effects of Jianpishugan particles are proved in STZ model mice,and may be realized via the antioxidant effects,by regulating expression of GLP-1 and FINS,glucagon levels,which should be further investiated.

关 键 词：健脾疏肝颗粒 糖尿病 抗氧化作用 GLP-1 

分 类 号：R943[医药卫生—药剂学]