丁螺环酮和米安色林对梁氏情境应激箱诱导小鼠焦虑和抑郁样行为的影响  

Effect of buspirone and mianserine on anxiety-and depression-like behaviors induced by Liang′s contextual-stress box in mice

作　　者：梁慧[1] 宫琦[1] 程涛[2] 梁建辉[1,2] 

机构地区：[1]北京大学基础医学院药理学系,北京100191 [2]北京大学药学院分子与细胞药理学系,北京100191

出　　处：《中国药理学与毒理学杂志》2017年第2期131-137,共7页Chinese Journal of Pharmacology and Toxicology

基　　金：十二五科技支撑计划项目(2011BAK04B08)~~

摘　　要：目的设计和建立梁氏情境应激箱,通过观察抗焦虑和抗抑郁药对小鼠行为学指标的影响,评价梁氏情境应激箱对小鼠焦虑和(或)抑郁样情绪反应的诱导作用。方法梁氏情境应激箱由中央区和3个外周臂构成。ICR小鼠分别ip给予抗焦虑药物丁螺环酮(0.5和1.0 mg·kg^(-1))和抗抑郁药物米安色林(0.25和0.5 mg·kg^(-1)),观察小鼠首次进入中央区的潜伏期、中央区停留时间、中央区活动路程、外周区活动路程、区域间穿梭次数和总活动度。结果 1丁螺环酮0.5和1.0 mg·kg^(-1)明显缩短小鼠首次进入中央区的潜伏期,分别缩短了71.9%(P<0.05)和77.9%(P<0.05);剂量依赖性地增加中央区停留时间,各增加了59.5%和73.8%(P<0.05);丁螺环酮0.5和1.0 mg·kg^(-1)均可显著减少小鼠区域间穿梭次数(P<0.05),但对其中央区活动路程、外周区活动路程和总活动度无明显影响;2米安色林0.25和0.5 mg·kg^(-1)明显缩短小鼠首次进入中央区的潜伏期,分别缩短了72.4%(P<0.05)和82.3%(P<0.05);剂量依赖性地增加中央区停留时间,各增加了39.1%和43.9%(P<0.05);米安色林剂量依赖性地增加中央区活动路程(P<0.05)及区域间穿梭次数(P<0.05),但对外周区活动路程和总活动度无明显影响。结论梁氏情境应激箱对小鼠焦虑和(或)抑郁样情绪反应具有明确的诱导作用,可作为一个新的焦虑抑郁共病动物模型研究其病理生理机制,筛选和评价新型抗焦虑和(或)抗抑郁药物。OBJECTIVE To investigate the effect of anxiolytics and antidepressants on anxietyand/or depression-like behaviours induced by designing a Liang′s contextual-stress box,（Liang′s box）in mice.METHODS Liang′s box was composed of a central area and three peripheral arms.Buspirone（0.5 and 1.0 mg·kg^-1） and mianserin（0.25 and 0.5 mg·kg^-1） were injected to mice intraperitoneally and the following behavioral indexes were recorded： ① latency to the central area（CA）, ②CA-time, ③ CAdistance, ④ distance traveled in the peripheral area（PA-distance） 5 number of the transitions, and 6global activity.RESULTS ① Buspirone 0.5 and 1.0 mg·kg^-1 decreased the latency to CA by as much as 71.9%（P〈0.05） and 77.9%（P〈0.05）, but dose-dependently increased the CA-time by 59.5% and73.8%（P〈0.05） respectively.Although buspirone 0.5 and 1.0 mg·kg^-1obviously reduced the shuttle number（P〈0.05）, it did not have significant pharmacological effects on the CA-and PA-distance or global activity.② Mianserin 0.25 and 0.5mg·kg^-1decreased the latency to CA by as much as 72.4%（P〈0.05）and 82.3%（P〈0.05）, but dose-dependently increased the CA-time by 39.1% and 43.9%（P〈0.05）, respectively.Mianserin enhanced the shuttle number（P〈0.05） and CA-distance（P〈0.05） in a dose-dependent manner, but it had no obvious pharmacological effects on the PA-distance or global activity.CONCLUSION Anxiety-and/or depression-like behaviors can be stimulated by Liang′s box in mice, which may be used as a novel animal model of anxiety and depression comorbidity to study its pathophysiological mechanisms, screen and evaluate certain new anxiolytics and antidepressants.

关 键 词：情境应激 焦虑 抑郁 共病现象 动物模型 

分 类 号：R965[医药卫生—药理学]