冲击波对人骨髓间充质干细胞增殖和成骨分化作用的研究  

Effects of Shock Wave on the Proliferation and Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells

作　　者：杨以萌 张浩[1] 廖伟雄[1] 苏祥正 李冀[1] 王克涛[1] 刘元林[2] 毛宁[2] 张毅[2] 张宁 张恭谦 朱恒[2] 李众利[1] 

机构地区：[1]中国人民解放军总医院骨科,运动医学中心,北京100039 [2]军事医学科学院基础医学研究所细胞生物学研究室,北京100850 [3]火箭军总医院骨科,北京100088 [4]青岛世纪杰创医疗科技有限公司,山东青岛266000

出　　处：《中国实验血液学杂志》2017年第1期209-213,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金面上项目(81371945,81572159,31370916);

摘　　要：目的:探讨冲击波对人骨髓间充质干细胞(MSC)增殖和成骨分化能力的影响。方法:从健康志愿者骨髓中分离培养骨髓间充质干细胞,将其分为3组,空白对照组细胞用普通培养液培养,成骨诱导组细胞用经典成骨诱导剂培养,冲击波组细胞用普通培养液培养,外加冲击波刺激。刺激后在镜下观察MSC形态;应用CCK-8检测冲击波组细胞和空白对照组MSC增殖能力的变化;第14天和28天分别用碱性磷酸酶和Von Kossa染色检测各组细胞的成骨能力。结果:CCK-8检测发现,冲击波与空白对照组相比,细胞的增殖活性显著增强;碱性磷酸酶和Von Kossa染色结果表明,冲击波组细胞的成骨分化能力比成骨诱导组要高;空白对照组细胞染色呈现弱阳性。结论:冲击波能促进骨髓间充质干细胞的增殖能力和成骨分化能力。Objective：To explore the effects of the shock wave on the capacity of mesenchymal stem cells（MSCs）to proliferate and differentiate into osteoblasts.Methods：MSCs were isolated from the bone marrow of healthy donors.The human bone marrow MSCs（BM-MSCs） were divided into 3 groups including blank control group,osteoinduced group and shock wave group.The MSCs in blank control group were cultured with common mediam;the MSCs in osteoinduced group were treated with osteogenic agents and cultured;the MSCs in shock wave group were cultured with common medium and stimulated by shock wave.The morphology of MSCs in each groups were observed by micoscopy;the CCK-8 was used to detect the proliferation ability of MSCs;the alkaline phosphatase staining and von Kossa staining were used to evaluale the differentiation potential of MSCs in each groups.Results：The results of CCK-8 revealed the shock wave could promote cell proliferation as compared with blank control group.The results of alkaline phosphatase and Von Kossa staining showed that the shock wave displayed a stronger ability to promote the human BMMSC differentiation into osteoblasts cells in comparison with the osteoinduced group.The blank control group was weakly positively stainined.Conclusion：The shock wave treatment can promote proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.

关 键 词：冲击波 骨髓间充质干细胞 细胞增殖 成骨分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]