miRNA-140-5p在人骨髓间充质干细胞成脂分化中的表达及其靶基因的预测  

Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation

作　　者：汪涛[1,2] 颜瑞巧[3] 曹俊 曹玲玲[4] 张铉浦 李兴暖 吴萍 周小鸥[1,2] 吴建芳[1,2] 许晓源 

机构地区：[1]江西省系统生物医学重点实验室,江西九江332000 [2]江西省九江学院基础医学院病原生物学教研室,江西九江332000 [3]江西省九江学院附属医院临床技能中心,江西九江332000 [4]江西省南昌大学附属九江医院内分泌科,江西九江332000

出　　处：《南方医科大学学报》2017年第2期199-203,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81460221;81260283;81160113);江西省自然科学基金(20161BAB205197)~~

摘　　要：目的筛选出人骨髓间充质干细胞(h MSCs)成脂分化中具有潜在价值的mi RNA及其靶基因,为人类成骨细胞与脂肪细胞分化平衡调控机制的深入研究提供有力实验依据。方法收集h MSCs成脂分化过程中的0、7、14、21 d的细胞样品,应用mi RNA芯片和转录组测序(m RNA-seq)技术,分析4个点细胞中mi RNA和m RNA的表达水平,并将mi RNA与m RNA的表达趋势进行关联分析,聚焦具有研究价值的mi RNA与m RNA相互调控关系,同时,采用Target Scan、Pic Tar和mi Randa等数据库进行靶基因的预测。结果发现mi R-140-5p在成脂分化中表达量呈逐渐下降的趋势,而白血病抑制因子受体(LIFR)表达量呈逐渐升高趋势,二者呈现负调控的对应关系。同时经3个靶基因数据库预测,LIFR为mi R-140-5p共有的靶基因。结论 mi RNA-140-5p可能与人骨髓间充质干细胞的成脂分化密切相关,且通过调控其靶基因LIFR的表达而参与成脂分化。Objective To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells （hMSCs） to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation. Methods Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing （RNA-seq） techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA. Results The expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor （LIFR） expression increased progressively during adipogerfic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p. Conclusion miRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.

关 键 词：人骨髓间充质干细胞 成脂分化 MI RNA-140-5p 白血病抑制因子受体 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]