低氧环境下丙泊酚可增加PC12细胞的凋亡  

Effect of propofol on apoptosis of PC12 cells under hypoxic condition and the mechanism

作　　者：孙茫[1] 沈炼桔[2] 刘阳[1] 于晴[1] 龙春兰[2] 李上莹莹 涂生芬[1,3,4] 

机构地区：[1]重庆医科大学附属儿童医院麻醉科,重庆400014 [2]重庆医科大学附属儿童医院儿科学重庆市重点实验室,重庆400014 [3]重庆医科大学附属儿童医院儿童发育重大疾病国家国际科技合作基地,重庆400014 [4]重庆医科大学附属儿童医院儿科发育疾病研究教育部重点实验室,重庆400014

出　　处：《南方医科大学学报》2017年第2期216-220,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(31200853);重庆市自然科学基金(cstc2012jj A10036);重庆市卫计委高端人才后备项目(2015HBRC007);国家临床重点专科建设项目(国卫办医函[2013]544)~~

摘　　要：目的探讨低氧环境下丙泊酚对PC12细胞株凋亡的影响及机制。方法将PC12细胞接种于培养板中,采用随机数字表法,将其随机分为6组:低氧对照组(CH组)、空气对照组(CA组)和氧气对照组(CO组),对照组加入脂肪乳浓度10μmol/L。丙泊酚低氧组(PH组)、丙泊酚空气组(PA组)和丙泊酚氧气组(PO组),丙泊酚浓度为10μmol/L。药物处理完毕后分别放入低氧(5%O_2),空气和氧气(35%O_2)环境的细胞培养箱培养,8 h后流式检测细胞凋亡情况,检测细胞内ROS水平和细胞SOD酶活力。结果与CA组比较,PA组细胞凋亡增加,活性氧簇(ROS)升高,超氧化物歧化酶(SOD)活力增强;与CH组比较,PH组细胞凋亡增加,ROS升高,SOD活力增强;与CO组比较,PO组细胞凋亡增加,ROS升高,SOD活力增强;与PA组比较,PH细胞凋亡增加,ROS升高,SOD活力增强;CO组、CA组和CH组上述各指标差异无统计学意义。结论低氧环境下丙泊酚可通过氧化应激损伤导致PC12细胞凋亡增加。Objective To investigate the mechanism by which propofol exposure causes PC12 cell apoptosis under hypoxic conditions. Methods PC12 cells were exposed to room air, 35% oxygen, or 5% oxygen （hypoxia） for 24 h in the presence of either 10 gmol/L lipid emulsion or 10 μmol/L propofol. After the treatments, the cell apoptosis was measured by flow ceytometry and the level of reactive oxygen species （ROS） and the activity of superoxide dismutase （SOD） were evaluated. Results In room air, PC12 cells treated with propofol showed increased apoptosis rate and ROS production as compared with the ceils treated with the lipid emulsion; propofol treatment of the cells exposed to 35% oxygen showed obvious enhancement of the apoptosis rate, ROS production and SOD activity. Under the hypoxic condition, propofol treatment even further increased the apoptosis rate, ROS production and SOD activity. Lipid emulsion caused no such changes in cells exposed to room air, 35% oxygen or 5% oxygen. Conclusion Under hypoxic conditions, propofol can cause apoptosis in PC12 cells by inducing oxidative stress injury.

关 键 词：丙泊酚 低氧 氧化应激 PC12 凋亡 

分 类 号：R614[医药卫生—麻醉学]