龙脷叶质量控制研究  

Study on Quality Control of Sauropus rostratus Miq.

作　　者：李兵[1] 曾艳婷[1] 莫惠雯 陈圣斌[1] 卢汝梅[1] 

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《亚太传统医药》2017年第5期24-26,共3页Asia-Pacific Traditional Medicine

基　　金：国家科技支撑计划项目(2013BAI11B06);广西科技厅重大专项计划项目(桂科重1355001-4-1)

摘　　要：目的:通过HPLC法测定龙脷叶中咖啡酸的含量,为龙脷叶药材质量控制提供分析方法。方法:采用HPLC法,色谱柱为Phenomenex ODS Hypersil C_(18),流动相为乙腈-0.1%磷酸(13∶87),检测波长为323nm。结果:咖啡酸在0.024~0.600mg·mL^(-1)范围内呈现良好的线性关系(r=0.999 9),方法学验证结果均符合相关要求。结论:该方法简单准确重复性好,为建立龙脷叶药材质量标准提供了依据。Objective ： To provide scientific analytical method for the quality control of Sauropus rostratus Miq. on content of caffeic acid. Methods： The content of eaffeic acid was determined by HPLC. The chromatographic conditions were as follows：Phenomenex ODS Hypersil C18 column was used with mobile phase consisted of aeetonitrle-0.1% phosphoricacid（13 ： 87）, and the detection wavelength was 323nm. Results： Caffeic acid content had a good linearity in the range of 0. 024-0. 600mg · mL-1 （r=0. 999 9）, the result of methodology validation meets the requirement. Conclusion ： The method is simple, feasible, and good repetitiveness, can be used for the quality control of Sauropus rostratus Miq.

关 键 词：龙脷叶 质量控制 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]