Box-Behnken响应面优化分光光度法测定瓜蒌总皂苷的显色条件  

Optimizing color conditions of spectrophotometric determination of total saponins in Fructus Trichosanthis with Box-Behnken response surface method

作　　者：徐启祥 鄢海燕[1] 阮玲[1] 陈倩[1] 邹纯才[1] 

机构地区：[1]皖南医学院药学院,芜湖241002

出　　处：《国际药学研究杂志》2017年第1期65-69,共5页Journal of International Pharmaceutical Research

基　　金：安徽高校省级自然科学研究重大项目(KJ2015ZD41;KJ2016SD60);安徽省科技攻关项目(12010402089)

摘　　要：目的优化分光光度法测定瓜蒌总皂苷的显色条件。方法研究影响瓜蒌总皂苷含量测定的各因素,包括显色温度、显色时间、5%香草醛-冰乙酸用量及高氯酸用量,并通过Box-Behnken响应面分析法优化分析条件。结果显色温度为60℃,显色时间为25 min,5%香草醛-冰乙酸用量为0.1 ml,高氯酸用量为0.6 ml时,显色稳定,测定结果准确。结论 BoxBehnken响应面法确定的显色条件可用于瓜蒌总皂苷的含量测定。Objective To optimize the color conditions of spectrophotometric determination of total saponins in Fructus Trichosanthis. Methods Several factors affecting the content determination of total saponins in Fructus Trichosanthis were studied,including the color temperature,color time,5% vanillin-acetic acid dosage,as well as the amount of perchloric acid. The analysis conditions were optimized by Box-Behnken response surface method. Results The optimized conditions were：temperature 60℃,the color time 25 min,5% vanillin-acetic acid 0.1 ml and perchloric 0.6 ml. The color was stable and the determination results were accurate.Conclusion The color conditions determined by Box-Behnken response surface method can be used for content determination of total saponins in Fructus Trichosanthis.

关 键 词：Box-Behnken响应面法 瓜蒌 总皂苷 分光光度法 

分 类 号：R284.1[医药卫生—中药学]