混合皮肤移植中自体角朊细胞表达B7-H1诱导免疫抑制的相关研究  

Related research of immunosuppression in intermingled skin transplantation induced by B7-H1 expressed on auto-keratinocytes

作　　者：蔡伟栋[1] 柏莹[1] 祝先进[1] 郑培烝[1] 曹颖平[1] 周光炎[2] 

机构地区：[1]福建医科大学附属协和医院检验科,福州350001 [2]上海市免疫学研究所,上海200025

出　　处：《中国免疫学杂志》2017年第2期174-177,185,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(81171656)

摘　　要：目的:探讨自体角朊细胞表达的B7-H1分子在混合皮肤移植中的作用和相关机制。方法:体外模拟混合皮肤移植模拟系统(MELC体系),通过流式细胞术检测角朊细胞B7-H1和淋巴细胞PD-1的表达。同时,实时荧光定量PCR检测淋巴细胞IL-10、Foxp3、GATA-3 mRNA的表达。结果:流式细胞仪检测结果显示,在有自体角朊细胞参与的MELC体系中,随着作用时间增加,角朊细胞上B7-H1的表达和淋巴细胞上PD-1的表达水平均明显升高,且具有时间依赖性(P<0.01);实时荧光定量PCR检测结果显示,随着作用时间增加,与无自体角朊细胞参与的MELC体系相比,在自体角朊细胞参与的MELC体系中,淋巴细胞IL-10、GATA-3、Foxp3 mRNA的表达水平均明显升高,且具有时间依赖性(P<0.01)。结论:在体外混合皮肤移植模拟系统中,自体角朊细胞通过表达共刺激分子B7-H1,上调淋巴细胞表面PD-1的表达,二者相结合,诱导Th2细胞和Foxp3+的Treg细胞分化,从而负向调节免疫反应。Objective： To explore the role and mechanism of the B7-H1 expressed by auto-keratinocytes in the intermingled skin grafting model in vitro（ MELC）. Methods： The intermingled skin grafting model（ MELC） was established in vitro. Flow cytometry was performed to detect the expressions of B7-H1 in keratinocytes. The expressions of PD-1 in lymphocytes were measured at the same time. The levels of IL-10,Foxp3 and GATA-3 mRNA in lymphocytes were detected by Real-time quantitative PCR. Results： Through flow eytometry,in the MELC with auto-keratinocytes,the expression of B7-H1 in auto-keratinocytes and the PD-1 in lymphocytes were rising trend and the rising rate was in time-dependent manners（ P〈 0. 01）. RT-PCR assay indicated that the relative levels of IL-10,Foxp3,GATA-3mRNA expression were significant raised and the rising rate was in time-dependent manners（ P〈0. 01）. Conclusion：In the intermingled skin grafting model,the auto-keratinocytes could express B7-H1 to enhance the expression of PD-1 in T cells. When B7-H1 combined with PD-1,the Th2 cells and Foxp3＋Tregs were induced and suppressed the immune response in the intermingled skin grafting model.

关 键 词：角朊细胞 B7-H1 混合皮肤移植 免疫抑制 

分 类 号：R392.4[医药卫生—免疫学]