电脉冲法增强CZP3 DNA疫苗免疫不育效果的研究  

Efficacy of CZP3 DNA vaccine immune infertility enhanced by electric pulse method

作　　者：王颖[1] 张贝贝[1] 阿地拉·艾皮热 张冀[1] 罗娇娇[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《中国免疫学杂志》2017年第2期237-241,共5页Chinese Journal of Immunology

基　　金：新疆维吾尔自治区科技支疆项目(No.201591134)

摘　　要：目的:研究电脉冲法对CZP3 DNA疫苗免疫不育效果的影响。方法:在小鼠腿部肌肉分别注射5、10、20和50μg的pc DNA3.0-CZP3质粒,经电脉冲刺激,ELISA法检测免疫小鼠的CZP3抗体水平及最高抗体滴度。与雄鼠合笼三周后,计算各免疫组的不孕率及平均产仔数,统计学分析。结果:相比单独肌肉注射免疫,电脉冲法能极显著提高DNA疫苗的免疫水平,电脉冲50μg组的抗体效价可以达到1∶4 000。此外随着血清中CZP3抗体水平的增高,小鼠不孕率明显上升,平均产仔数显著减少。统计分析显示,免疫小鼠的CZP3抗体水平与产仔数呈负相关关系。结论:电脉冲法能够显著提高CZP3 DNA疫苗的免疫水平,降低小鼠生育能力,这为CZP3 DNA疫苗应用于流浪犬避孕奠定了基础。Objective： To analyze the immunocontraceptive efficacy of CZP3 DNA vaccine by electric pulse stimulating and the correlation between antibody level and antifertility. Methods： The mice were immunized with 5,10,20 and 50 μg DNA vaccine pc DNA3. 0-CZP3 respectively and were stimulated at leg muscle by electric pulse. Detect the antibody level of each mice group and the highest antibody titer by ELISA. After mated with male mice for three weeks,calculated infertility rate of mice,the average litter size of each immune group and did statistical analysis. Results： Compared with the intramuscular injection alone,the electric pulse stimulation could increase serum antibody titers of mice significantly,the antibody titer of 50 μg group which was stimulated by electric pulse reached 1 ∶4 000. Moreover,the infertility of the mice increased and the mice average litter size reduced along with the increase of CZP3 antibody level significantly. Statistical analysis showed that the antibody level and the average litter size had a negative correlation. Conclusion： The electric pulse stimulation can improve the immune level and reduce the fertility efficiency of mice significantly,therefore establishes a foundation for further application of CZP3 DNA vaccine in stray dog contraception.

关 键 词：卵透明带3 DNA疫苗 电脉冲法 免疫不育 

分 类 号：Q819[生物学—生物工程] R392-33[医药卫生—免疫学]