丹酚酸A对慢性肾衰竭大鼠BMP-7/Smads/TGF-β1信号通路的调控作用  

The Regulating Role of Salvianolic Acid A on BMP-7/Smads/TGF-beta 1 Signaling Pathway in Chronic Renal Failure Rats

作　　者：唐英[1] 何立群[1] 朱祎[2] 张翼[2] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021 [2]上海中医药大学,上海200021

出　　处：《中国中西医结合肾病杂志》2017年第1期9-13,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国家“重大新药创制”专项项目(No.2009ZX09311-003);国家中医药管理局重点学科;上海市中医药事业发展三年行动计划项目(No.ZYSNXD-CC-YJXYY)

摘　　要：目的:观察丹酚酸A对5/6肾切除(Platt法)大鼠肾组织骨形态发生蛋白-7(BMP-7)、转化生长因子β_1(TGF-β_1)/Smads信号通路的调节作用。方法:将40只大鼠随机分为:假手术组、模型组、丹酚酸A组、科素亚组,采用Platt法复制慢性肾衰竭大鼠模型,术后8周分别观察大鼠肾组织BMP-7、Smad6、TGF-β_1蛋白及基因的表达,p-Smad2/3、p-Smad1/5/8的蛋白表达。结果:各治疗组大鼠肾组织BMP-7、Smad6、p-Smad1/5/8的表达较模型组升高(P<0.01 or P<0.05),TGF-β_1、p-Smad2/3的表达较模型组明显减弱(P<0.01 or P<0.05)。结论:丹酚酸A可能是通过诱导BMP-7、Smad6、p-Smad1/5/8蛋白表达,抑制p-Smad2/3蛋白表达,调节BMP-7/Smads/TGF-β_1信号通路,抑制了TGF-β_1信号向细胞核内转导的通路,从而抑制细胞外基质增生,起到延缓肾纤维化的作用。Objective ：To study the adjustment of Salvianolic acid A on BMP 7 and TGF - beta 1/Smads signal pathways in 5/6 nephrectomy （Platt method） in the rat kidney. Methods：Divided the SD rats randomly into sham group and model group, SAA group and Cozaar group. The rats were established CRF animal model by Platt method. After 8 weeks of treatment, Detect the the protein and gene expression of BMP7, smad6 and TGF - β1 in kidney tissues. Detect the protein expression of p - smad2/3 p - smadl/ 5/8 in kidney tissue in each group. Results： Compared with the model group, the expression of BMP - 7, Smad6, P - Smadl/5/8 were increased （P 〈 0. 01 or P 〈 0. 05 ） , expression of TGF - [31 , P - Smad2/3 were decreased （ P 〈 0.01 or P 〈 0.05 ）. Conclusion ： SAA play a role of antifibrotic effects may be by increasing the expression of BMP - 7, Smad6, P - Smadl/5/8 in rats with chronic renal failure, and decreasing the expression of P -Smad2/3, affecting the BMP -7/Smad/TGF - β1 signaling pathways to inhibite TGF - β1 signal transducting to the nucleus; then to inhibite the proliferation of extracellular matrix and to delay the renal fibrosis.

关 键 词：肾纤维化 丹酚酸A BMP-7/Smads/TGF-β1 

分 类 号：R285.5[医药卫生—中药学]