百脉根组氨酸蛋白激酶LHK1的表达及活性鉴定  

Expression and activity of lotus histidine kinase 1 in Lotus japonicus

作　　者：张恒 尹珺[1] 张晴[1] 张忠明[1] 

机构地区：[1]华中农业大学生命科学技术学院/农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2017年第2期59-63,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31370278);国家重点基础研究发展计划"973"项目(2010C126502)

摘　　要：为研究LHK1蛋白的功能,构建了pPub-LHK1∶AG-GFP和pFastBac-LHK1表达载体,分别在烟草叶肉细胞和昆虫细胞表达百脉根组氨酸蛋白激酶LHK1跨膜蛋白。结果表明:在烟草和昆虫细胞中LHK1蛋白都能表达,且表达的蛋白在体外都能磷酸化底物(组氨酸转移酶HP1蛋白),具有组氨酸蛋白激酶活性。Lotus histidine kinase 1is a cytokinin receptor protein which serves an important function in symbiotic signaling pathway and nodules formation.Two sets of eukaryotic expression vectors were built and expressed in tobacco expression system and baculovirus insect expression system to study the function of LHK1 protein.The results showed that it can be expressed in tobacco and insect cells.The LHK1 protein expressed has histidine protein kinase activity in vitro.It will be useful for further studying the biochemical mechanism of LHK1 in symbiotic signaling pathway.

关 键 词：百脉根 组氨酸蛋白激酶 烟草表达系统 昆虫表达系统 

分 类 号：Q939.9[生物学—微生物学]