陆地棉幼苗NaCl胁迫下转录因子的转录组学分析  

Transcriptome Analysis of Transcription Factors in Upland Cotton Seedlings under NaCl Stress

作　　者：彭振[1,2] 何守朴[1] 龚文芳[1] 潘兆娥[1] 贾银华[1] 卢艳丽[2] 杜雄明[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000 [2]四川农业大学玉米研究所,四川温江611130

出　　处：《作物学报》2017年第3期354-370,共17页Acta Agronomica Sinica

基　　金："十二五"国家科技支撑计划项目(2013BAD01B03);国家自然科学基金项目(31301365)资助~~

摘　　要：以棉花耐盐种质早熟长绒7号和感盐种质南丹巴地大花为材料,利用转录组测序(RNA-Seq)技术分析2个种质在200 mmol L–1 Na Cl胁迫4 h和24 h后幼苗叶片的转录因子家族及转录因子的表达情况。结果表明,从样本中鉴定出54个转录因子家族的2815个转录因子。盐胁迫4 h后,长绒7号有249个转录因子基因表达发生变化;南丹巴地大花中有261个转录因子基因表达发生变化。在胁迫24 h后,2个种质对盐响应的转录因子数量均剧增。只在耐盐种质长绒7号特异表达的有106个(4 h)和184个(24 h)转录因子基因,对于2个种质共同表达的转录因子有143个(4 h)和282个(24 h)。通过筛选,鉴定出26个与耐盐相关的转录因子家族124个转录因子基因,并采用荧光定量验证转录组数据的准确性。推测高表达的11个转录因子的基因CotAD_66280(HD-ZIP)、CotAD_47058(ERF)、CotAD_18472(G2-like)、CotAD_04289(C2H2)、CotAD_57763(HSF)、CotAD_23656(TCP)、CotAD_02221(ERF)、CotAD_23975(WRKY)、CotAD_54036(MYB)、CotAD_27788(NAC)和CotAD_23815(HSF)有可能与陆地棉抗盐性密切相关,可作为陆地棉耐盐育种的候选基因源。A comparative transcriptomics was used to analyze the expression changes of transcription factors and their families in leaves of the two cotton（Gossypium hirsutum L.） varieties（Earlistaple 7 with salt-tolerance and Nandanbadidahua with salt-sensitivity） under 200 mmol L^（–1） NaCl stress for 4 and 24 hours.We have identified and quantified 2815 transcription factors from 54 transcription factor families of the samples.A total of 249 differentially expressed transcription factor genes of ‘Earlistaple 7＇ and 261 transcription factor genes of ‘Nandanbadidahua＇ were identified,after 4 hours of NaCl stress.With prolonging stress time（24 hours）,the number of differentially expressed transcription factors in two cotton varieties responsive to salt was increased.It was found that the 106（4 hours） and 184（24 hours） transcription factor genes were specially expressed in salt-tolerant variety,and the 143（4 hours） and 282（24 hours） were exhibited commonly in both cotton lines.We have obtained 124 salt-tolerance-related transcription factor genes from 26 transcription factor families through screening,and further validated the data accuracy by quantitative Real-time PCR.Total 11 highly expressed transcription factors genes by salt induction,including CotAD_66280（HD-ZIP）,CotAD_47058（ERF）,CotAD_18472（G2-like）,CotAD_04289（C2H2）,CotAD_57763（HSF）,CotAD_23656（TCP）,CotAD_02221（ERF）,CotAD_23975（WRKY）,CotAD_54036（MYB）,CotAD_27788（NAC）,and CotAD_23815（HSF） were induced to be closely related to salt tolerance in upland cotton,and might be used as a source of candidate tolerant genes for upland cotton breeding.

关 键 词：陆地棉 NACL胁迫 转录组分析 转录因子 

分 类 号：S562[农业科学—作物学] Q943.2[生物学—植物学]