HCl和DMSO阻断滞育条件下桑蚕卵壳电镜观察  

SEM observation on silkworm eggshell under HCl or dimethyl sulfoxide(DMSO) treatment for preventing diapause

作　　者：龚竞[1] 田莎[1] 侯勇[1] 赵欢欢[1] 朱勇[1] 

机构地区：[1]西南大学生物技术学院,重庆400716

出　　处：《华南农业大学学报》2017年第2期1-5,共5页Journal of South China Agricultural University

基　　金：973计划(2012CB114600);国家自然科学基金(31201853);重庆市基础与前沿研究计划(cstc2015jcy A0469)

摘　　要：【目的】探讨桑蚕Bombyx mori滞育阻断机理,比较HCl、二甲基亚砜(DMSO)法阻断蚕卵滞育后卵壳表面发生的一系列变化。【方法】用HCl和DMSO分别在产后20和12 h处理蚕卵,利用扫描电镜观察卵壳表面及气孔在处理后不同时间点的变化。【结果】HCl和DMSO处理均能成功地阻断蚕卵滞育的发生,蚕卵平均孵化率分别为93.37%和87.81%。扫描电镜观察HCl和DMSO处理的卵壳表面蚀刻效果明显,均出现了"脂质"脱落物;并且处理后蚕卵的气孔有逐步增大的趋势,与DMSO处理相比较,HCl处理获得的现象更为明显。【结论】卵壳表面变化可能与桑蚕滞育的阻断有关。【Objective】To investigate the diapause prevention mechanism of silkworm（ Bombyx mori）,and compare a series of changes of eggshell surface after diapause prevention by HCl or DMSO treatment.【Method】The eggs were treated with HCl or DMSO at 20 and 12 h after oviposition respectively. Scanning electron microscope（ SEM） was used to observe the changes of eggshell surface and aeropyle at different time points after treatment. 【Result】Both HCl and DMSO treatments successfully prevented the occurrence of egg diapause,and the average hatching rates were 93. 37% and 87. 81% respectively.SEM observation showed clear etching effect and ＂lipid ＂shed on eggshell surface,and the aeropyle diameter increased gradually under both treatments. The above observations were more obvious for the HCl treatment compared with the DMSO treatment. 【Conclusion】Eggshell surface changes may be related to the diapause prevention of silkworm.

关 键 词：桑蚕 阻断滞育 HCL DMSO 扫描电镜 卵壳 

分 类 号：S828[农业科学—畜牧学]