光动力学疗法对瘢痕成纤维细胞中转化生长因子β_1的作用  

The Investigation of Transforming Growth Factor β_1 in HSF after HMME-PDT

作　　者：蔡宏[1] 王毅侠[1] 李铀[1] 刘玮[1] 孙平[1] 董宁[2] CAI Hong WANG Yi-xia LI You LIU Wei SUN Ping DONG Ning(Department of Dermatology, The General Hospital of Air Force, Beijing 100142, China Burns Institute, the First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100037,China)

机构地区：[1]解放军空军总医院皮肤科,北京100142 [2]解放军总医院第一附属医院烧伤研究所,北京100037

出　　处：《中国美容医学》2017年第2期5-8,共4页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助(光动力学疗法作用于瘢痕疙瘩成纤维细胞TGF-β1/Smad3信号通路的分子机制:编号81301386)

摘　　要：目的:探讨光动力学疗法(Photodynamic therapy,PDT)对体外培养瘢痕成纤维细胞(hypertrophic scar fibroblast,HSF)中转化生长因子β_1的影响。方法:将原代培养的HSF分为对照组、HMME-PDT组、单纯光敏剂组和单纯激光照射组,用酶联免疫吸附法(ELISA)检测PDT后HSF分泌至上清液的TGF-β_1蛋白表达量,应用Western-Blot观察PDT对细胞浆内转化生长因子β_1的作用。结果:HSF分泌至细胞上清液的TGF-β_1蛋白含量较高,而HMME-PDT后降低;HSF合成的TGF-β_1蛋白水平高,HMME-PDT后HSF中的TGF-β_1蛋白含量减少。结论:HMME-PDT能够阻止TGF-β_1的信号传向细胞核,改变TGF-β_1在HSF细胞水平的表达,使细胞核内细胞增殖及胶原合成有关的靶基因得不到激活,从而抑制HSF增殖。Objective To investigate the response of TGF-β_1 after PDT which induced by hematoporphyrin monomerthyl ether（HMME） in human fibroblasts from hypertrophic scar（HSF）. Methods Fibroblasts were cultured from nontreated hypertrophic scars, and cells were divided into 4 groups： control（untreated）, HMME-PDT, HMME and Laser. The expression of TGF-β_1 in supernatant of HSF was detected by enzyme linked immunosorbent assay（ELISA）. The expression of TGF-β_1 was analyzed by Western blot. Results HMME-PDT down-regulated the protein level of TGF-β_1 both in supernatant of HSF and in HSF. Conclusion HMME-PDT decreased TGF-β_1 activation. It inhibited the proliferation of HSF through decreased the activation of taget gene.

关 键 词：光动力学疗法 激光 光敏剂 瘢痕成纤维细胞 转化生长因子Β1 

分 类 号：R619.6[医药卫生—外科学]