HPA-shRNA对乳腺癌MDA-MB-231细胞的肿瘤抑制作用  

THE ANTI-TUMOR EFFECT OF HPA-SHRNA ON MDA-MB-231 BREAST CANCER CELLS

作　　者：陈国利[1] 郑志方[2] 程利民[1] 张学军[1] 

机构地区：[1]承德医学院附属医院外一科,河北省承德市067000 [2]承德医学院附属医院小儿内科,河北省承德市067000

出　　处：《中国煤炭工业医学杂志》2017年第2期190-194,共5页Chinese Journal of Coal Industry Medicine

基　　金：承德市科技支撑计划项目(编号:20151029)

摘　　要：目的探索乙酰肝素酶短发夹RNA(HPA-shRNA)对乳腺癌细胞的抗肿瘤作用。方法根据RNA干扰(interfering RNA,RNAi)序列设计原则,以乳腺癌HPA基因为靶基因,构建shRNA重组慢病毒表达载体,通过Western-blot检测HPA-shRNA的敲降作用;通过CCK8实验观察HPA-shRNA对乳腺癌MDA-MB-231细胞生长增殖的影响;运用Transwell实验检测HPA-shRNA对乳腺癌MDA-MB-231侵袭能力的影响。结果经测序分析证实HPA-shRNA1重组慢病毒载体构建成功;在MDA-MB-231细胞内,HPA-shRNA1有效降低了HPA蛋白的表达水平(P<0.05);通过体外实验证明了HPA-shRNA1能够抑制乳腺癌MDA-MB-231细胞的生长增殖及侵袭能力。结论 HPA-shRNA1重组慢病毒载体可有效抑制HPA在乳腺癌细胞中的表达,并且证实HPA-shRNA对MDA-MB-231乳腺癌细胞具有良好的抗肿瘤作用。Objective To explore whether the HPA-shRNA has the anti-tumor effect on breast cancer cells.Methods According to the interfering RNA sequence designed principle,short hairpin RNA targeting HPA gene（HPA-shRNA）recombinant lentivirus-based Vectors were constructed.The knowdown effect of HPA-shRNA was detected by Western-blot;then,cell viability was detected by CCK8 assay.The transwell assay was used to validate the function of HPA-shRNA in invision.Results HPA shRNA1 recombinant Lentivirus vectors was successfully constructed by sequencing analysis.The protein expression level of HPA of MDA-MB-231 cells of breast cancer could significantly be inhibited by HPAshRNA1（P〈0.05）;HPA-shRNA1 suppressed the proliferation and invision of MDA-MB-231 cells.Conclusion The results indicate that HPA-shRNA1 recombinant Lentivirus vectors successfully inhibits the expression level of HPA in human breast cancer cells,and HPA-shRNA has the anti-tumor activity in MDA-MB-231 breast cancer cells in vitro.

关 键 词：慢病毒载体 乙酰肝素酶 抗肿瘤 短发夹RNA MDA-MB-231细胞 

分 类 号：R655.8[医药卫生—外科学]