福州地区碳青霉烯类耐药鲍曼不动杆菌碳青霉烯酶基因型研究  

The genotypes of carbapenemases in carbapenem-resistant Acinetobacter baumannii in Fuzhou area

作　　者：施腾飞[1] 陈惠瑜[2] 刘银环[1] 高丽钦[3] 

机构地区：[1]厦门大学附属福州第二医院检验科,福建福州350007 [2]福建省妇幼保健院检验科,福建福州350001 [3]福建医科大学附属第一医院检验科,福建福州350004

出　　处：《中国微生态学杂志》2017年第2期155-158,共4页Chinese Journal of Microecology

基　　金：福州市卫生局青年科研项目资助(2013-S-W3)

摘　　要：目的探讨福州地区碳青霉烯类耐药鲍曼不动杆菌(CRAB)碳青霉烯酶基因型的流行情况。方法收集多家医院临床标本中分离得到的107株CRAB。应用K-B纸片扩散法进行药物敏感试验。采用PCR法检测7种碳青霉烯酶基因,包括OXA-23、OXA-24、OXA-51、OXA-58、IMP-1、IMP-4和VIM-2。结果 107株CRAB对除多粘菌素B、米诺环素外的其他所有常见的抗生素均为耐药。碳青霉烯酶基因OXA-51、OXA-23的检出率分别为100.0%(107/107)和87.9%(94/107)。其他OXA-24、OXA-58、IMP-1、IMP-4和VIM-2基因均未检出。结论福州地区临床分离的CRAB耐药现象严重;表达OXA-23基因是CRAB对碳青霉烯类药物耐药的重要机制之一。Objective To investigate the prevalence of carbapenemases genotypes in carbapenem-resistant Acinetobacter baumannii（CRAB）in Fuzhou area.Methods Totally 107 strains of CRAB were collected from a number of hospitals in Fuzhou.Drug sensitivity test was performed by using Kirby-Bauer diffusion method.PCR was used to detect seven kinds of carbapenemases genes,including OXA-23,OXA-24,OXA-51,OXA-58,IMP-1,IMP-4and VIM-2.Results The 107 strains of CRAB were resistant to almost all the common antibiotics except Polymyxin B and Minocycline.The detection rates of OXA-51 and OXA-23were100.0%（107/107）and 87.9%（94/107）,respectively.OXA-24,OXA-58,IMP-1,IMP-4and VIM-2genes were not detected.Conclusion The resistance of clinical CRAB isolates in Fuzhou area is high.The expression of OXA-23 gene is one of the important mechanisms of their drug resistance to carbapenems.

关 键 词：鲍曼不动杆菌 碳青霉烯酶 耐药基因 

分 类 号：R378[医药卫生—病原生物学]