Real-time PCR分析滴虫性阴道炎患者微生物组成的变化  

The microbiota changes in trichomonas vaginitis patients based on Real-time PCR analysis

作　　者：朱丽红[1] 张宁[2] 江滟[2] 程昊[1] 张彦[3] 

机构地区：[1]复旦大学附属华东医院妇科,上海200040 [2]贵州医科大学病原生物学系,贵州贵阳550004 [3]上海交通大学医学院免疫学与微生物学系,上海200025

出　　处：《中国微生态学杂志》2017年第2期207-210,共4页Chinese Journal of Microecology

基　　金：上海市公共卫生三年行动计划重点学科建设项目传染病与卫生微生物学(15GWZK0102)

摘　　要：目的探寻滴虫性阴道炎患者阴道微生物组成的特点,为临床诊治及减少疾病的复发提供参考依据。方法选取2016年5-6月复旦大学附属华东医院妇科门诊45岁以下确诊为滴虫性阴道炎的育龄期患者16例,以同期18例45岁以下育龄期健康女性为对照,采集患者和健康人上阴道壁1/3处的分泌物,进行常规临床分析和革兰染色,并以21种常见的阴道细菌的特异引物进行Real-time PCR检测,分析并比较这些细菌在滴虫性阴道炎患者和健康人阴道内的组成及变化。结果在18例健康人中,其中13例的阴道微生物组成以乳酸杆菌(Lactobacillus)为优势菌,3例的阴道微生物组成无明显优势菌群或者优势菌不为检测的21种常见阴道微生物,2例的阴道微生物组成以加德纳菌为优势菌;在16例滴虫性阴道炎患者中,只有2例患者的阴道微生物菌群以乳酸杆菌为优势菌,14例以厌氧菌如普雷沃属(Prevotella)、纤毛菌(Leptotrichia)和斯尼思菌(Sneathia)为优势菌。经Metastats进行组间差异分析,结果表明Lactobacillus crispatus、Leptotrichia amnionii、Eggerthella sp.和Peptostreptococcus sp.在两组之间差异有统计学意义(P≤0.05)。PCoA主成分析结果亦显示滴虫性阴道炎患者的阴道常见21种微生物组成与健康人有显著差别。结论滴虫性阴道炎患者阴道内微生物组成的多样性增加,普雷沃属、纤毛菌及斯尼思菌的明显增高可能滴虫性阴道炎的炎症发生有关,此结果对于临床治疗滴虫性阴道炎的及减少复发提供新的方向,具有一定的指导意义。Objective To explore the characteristics of vaginal microbiota in trichomonas vaginitis patients,in order to provide the basis for clinical diagnosis and treatment and reduce the recurrence of the disease.Methods 16 patients diagnosed as trichomonal vaginitis and 18 healthy women in outpatient department from May to June in2016 were enrolled.Vaginal secretion specimens were collected for Gram staining and routine clinical analysis.21 common vaginal bacteria were quantified by Real-time PCR using species-specific primer.Results In healthy group,vaginal Lactobacillus was dominant in 13 cases,Gardnerellain 2cases,and no dominant bacteria in another 3cases.In trichomoniasis group,Lactobacillus was detected in only 2cases;Prevotella spp.,Leptotrichia amnionii and Sneathia sanguinegens were the dominant species in 14 cases.Conclusion The diversity of microbial composition in Trichomonas vaginalis increased.Prevotellaspp.,Leptotrichia amnionii and Sneathiasanguinegens are most frequently found species in Trichomonas vaginitis.This result may provide a new direction for clinical treatment and control of trichomoniasis.

关 键 词：滴虫性阴道炎 阴道内环境 菌群构成 

分 类 号：R711.3[医药卫生—妇产科学]