联苯双酯对小鼠骨髓细胞辐射损伤的防护作用研究  

The protective effects of dimethyl dicarboxylate biphenyl on the mice bone marrow cells exposed to irradiation

作　　者：董辉[1] 侯沁莲 姜道利[1] 唐卫生[1] 田真源 周则卫[1] 李德冠[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所天津放射医学与分子核医学重点实验室,天津300192

出　　处：《中国医药导报》2017年第4期4-7,26,共5页China Medical Herald

基　　金：国家自然科学基金(81573094);天津市自然科学重点基金(15JCZDJC35200)

摘　　要：目的探讨联苯双酯对辐射致小鼠骨髓细胞损伤的防护作用。方法体外无菌分离小鼠骨髓细胞,联苯双酯孵育30 min后给予1 Gy照射,18 h后观察对骨髓细胞的细胞活力、细胞凋亡以及活性氧水平的影响。体内实验C57小鼠分为四组,对照组、联苯双酯组、照射组和照射给药组。其中照射组和照射给药组给予4 Gy全身照射,对照组和联苯双酯组给予假照射(0Gy)。联苯双酯组和照射给药组在受照后灌胃联苯双酯(450 mg/kg),持续给药7 d,对照组和联苯双酯组给予相同体积的生理盐水。在照射第9天取骨髓细胞,计数单侧股骨骨髓细胞数量以及观察骨髓细胞的凋亡、活性氧水平以及克隆形成能力。结果体外实验中,联苯双酯在5μmol/L有一定的保护作用。在给予1 Gy照射后18 h,与对照组相比,照射组细胞活力显著下降、凋亡率以及活性氧水平显著升高(P<0.05);与照射组比较,照射给药组中联苯双酯可提高受照骨髓细胞的细胞活力,降低骨髓细胞凋亡率和活性氧水平(P<0.05)。体内实验中,与对照组比较,照射组可显著降低骨髓细胞计数和克隆形成能力,提高骨髓细胞活性氧水平(P<0.05);与照射组比较,照射给药组中联苯双酯可显著改善骨髓细胞克隆形成能力,降低骨髓细胞活性氧水平。结论联苯双酯可降低骨髓细胞活性氧水平,对骨髓细胞辐射损伤有一定的防护作用。Objective To observe the protective effects of dimethyl dicarboxylate biphenyl （DDB） on the mouse bone marrow （BM） ceils in vivo and in vitro. Methods In vitro experiments,the bone marrow cells were isolated and exposed to 1 Gy irradiation after 30 min DDB treatment.Then the cell viabilities,reactive oxygen species （ROS） and apoptosis were detected after 18 h culture.In vivo experiments,the C57BL/C6 mice were divided into control group,irradiated group,DBB treated group and irradiated＋DBB group.The mice in the control and DBB group received sham irradiation, and the mice in the irradiated group and the irradiated＋DBB group accept 4 Gy total body irradiation. DBB was gavage administrated 450 mg/kg 7d to the mice in the DBB group and the irradiated＋DBB. The same volume PBS were given to the mice in the control and irradiated group.After 4 Gy irradiation 9 day, the BM nucleated cell were counted,the ROS,apoptosis and CFU-GM of BM cells were detected. Results In vitro experiments,5 ~xmol/L DBB showed protective effects.Compared to the control group,the BM cells received 1Gy irradiation 18h showed cell viability decreased,the apoptosis and ROS levels increased （P 〈 0.05）.The BM cells injuries induced by irradiation were relieved compared to the irradiated group （P 〈 0.05）.In vivo experiments,the BM cells counts and CFU-GM ability in irradiated mice were decreased,the ROS levels were increased compared to those of control group （P 〈 0.05）.The BM cells counts and CFU- GM ability was increased,the ROS levels were decreased by DBB treatment compared to those of irradiated group. Conclusion DBB showed protective effects on the bone marrow ceils irradiation injury by inhibiting ROS levels.

关 键 词：辐射 活性氧 联苯双酯 骨髓细胞 细胞凋亡 

分 类 号：R811.5[医药卫生—放射医学]