高胰岛素增加早孕小鼠子宫内膜蜕膜化进程中血管紧张素Ⅱ的表达  

High insulin increases expression of angiogenensis Ⅱ in the endometrium of early pregnant mice

作　　者：陈文琪[1] 张晨[1] 王应雄[1] 何俊琳[1] 刘学庆[1] 陈雪梅[1] 穆欣艺[1] 丁裕斌[1] 高茹菲[1] 

机构地区：[1]重庆医科大学公共卫生与管理学院生殖生物学研究室,重庆400016

出　　处：《基础医学与临床》2017年第3期341-345,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金青年基金(81300486);重庆市科委前沿与应用基础研究项目(cstc2015jcyj A10013);重庆市渝中区基础与前沿科技项目(20150104)

摘　　要：目的探讨高水平胰岛素对早孕小鼠子宫内膜蜕膜化进程中血管标志分子血管紧张素Ⅱ(Ang-Ⅱ)的影响。方法皮下注射胰岛素构建高水平胰岛素动物模型。将其与正常雄鼠交配后收集妊娠第6、7和8天(D6、D7和D8)的子宫组织及血清,并监测血糖。另将其与输精管结扎的雄鼠交配构建人工诱导蜕膜化模型,于假孕D8收集子宫组织。ELISA检测血清中胰岛素及雌、孕激素的变化,real-time PCR检测子宫内膜Ang-ⅡmRNA的表达,Western blot对子宫内膜Ang-Ⅱ蛋白表达进行检测。结果高胰岛素模型中血清胰岛素水平于妊娠D6、D7和D8均明显升高(P<0.001)、雌激素水平于妊娠D8出现显著下降(P<0.05),孕激素水平于妊娠D6、D8出现明显下降(P<0.01)。与对照组比较,高胰岛素模型中子宫内膜组织中Ang-Ⅱ的mRNA及蛋白的表达显著增加(P<0.05)。结论高胰岛素增加孕鼠蜕膜化进程中子宫内膜组织Ang-Ⅱ的表达,从而影响子宫内膜蜕膜化进程中的血管生成。Objective To investigate the effect of high level insulin on the vascular marker molecules Ang-Ⅱ in the endometrium of early pregnant mice. Methods The high insulin animal model was constructed by subcutaneous injection of insulin. The uterine tissues and serum from D6,D7,and D8 days of pregnancy which were collected after mating with normal male rats,and blood glucose was simultaneously monitored. In addition to the male rats with vasectomized mating to construct artificial decidualization. ELISA for the detection of serum insulin,progesterone,and estradiol changes,real time PCR to detect the Ang-Ⅱ mRNA expression in endometrial tissue. Western blot for the detection of Ang-Ⅱ protein expression in uterine tissue. Results Insulin in the high insulin group of pregnancy D6,D7,and D8 days increased significantly（ P 0. 001）,estrogen levels in pregnancy D8 days appear significantly decreased（ P 0. 05） and progesterone levels in pregnancy D6 and D8 days appear to decline significantly（ P 0. 01）. The expression of Ang-ⅡmRNA and protein significantly increased in the high insulin model（ P 0. 05）. Conclusions High insulin can increase the expression of Ang-Ⅱin the endometrium of pregnant rats during the process of pregnancy. It is suggested that insulin may may contribute to the angiogenesis in the endometrium of the uterus.

关 键 词：胰岛素 子宫内膜蜕膜化 Ang-Ⅱ 血管生成 

分 类 号：R714[医药卫生—妇产科学]