尼古丁促人脐静脉内皮细胞凋亡  

Nicotine promotes the apoptosis of human umbilical vein endothelial cells

作　　者：刘秀[1,2] 邱宸阳 王超楠[2] 刘暴[1] 无 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院血管外科,北京100730 [2]河北北方学院研究生部,河北张家口075000 [3]首都医科大学附属复兴医院血管外科,北京100037

出　　处：《基础医学与临床》2017年第3期391-395,共5页Basic and Clinical Medicine

摘　　要：目的研究不同浓度尼古丁对人脐静脉内皮细胞的促凋亡作用及作用机制。方法体外培养人脐静脉内皮细胞,根据尼古丁浓度分组为10-6、10-7和10-8mol/L组及对照组。尼古丁作用24 h后,用CCK-8法检测细胞增殖,Annexin V-FITC荧光双染色法检测细胞凋亡。Western blot检测Bax、Bcl-2和PARP-1蛋白表达,对其作用机制进行研究。结果与对照组相比,10-6、10-7mol/L尼古丁组细胞增殖活性下降(P<0.05);凋亡数目增多(P<0.01)。促凋亡蛋白Bax的表达量增加(P<0.01);抑凋亡蛋白Bcl-2表达明显减少(P<0.01);PARP-1表达增加(P<0.01)。结论一定浓度的尼古丁对血管内皮细胞具有促凋亡作用,加速动脉粥样硬化性疾病的发生发展。Objective To try different concentrations of nicotine promotes the apoptosis of human umbilical vein endothelial cells and its mechanism. Methods Human umbilical vein endothelial cells were cultured. According to the concentration of nicotine,grouped as 10- 6,10- 7,10- 8mol / L,and control. After 24 h of nicotine treatment,proliferation / activity were tested by CCK-8 assay; Annexin V-FITC fluorescence staining detects the apoptotic cells. Western blot detects the expression of protein Bax,Bcl-2 and PARP-1,to study the mechanism of apoptosis. Results Compared with control group,in 10- 6,10- 7mol / L groups the proliferation / activity of HUVECs decreased（ P 0. 05）. And the number of apoptotic cells increased（ P 0. 01）. The target proteins expression of10- 6、10- 7mol / L groups were changed as follow： Bax increased（ P 0. 01）,Bcl-2 decreased（ P 0. 01） and PARP-1 increased（ P 0. 01）. Conclusions Nicotine may promote the apoptosis of vascular endothelial cells,which accelerates the development of atherosclerotic disease.

关 键 词：人脐静脉内皮细胞 尼古丁 凋亡 

分 类 号：R54[医药卫生—心血管疾病]