神经梅毒患者脑脊液蛋白芯片分析  

Protein microarray analysis in cerebrospinal fluid of neurosyphilis patients

作　　者：吕萍[1,2] 方昶[3] 黄进梅[1,2] 曾仁山[4] 黄涛[1,2] 杨斌[1,2] 

机构地区：[1]南方医科大学皮肤病医院 [2]广东省皮肤病医院,广东广州510091 [3]中山大学孙逸仙纪念医院,广东广州510120 [4]广州市第一人民医院,广东广州510180

出　　处：《皮肤性病诊疗学杂志》2017年第1期3-7,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：广东省自然科学基金(编号:2015A030310070);广东省医学科研基金(编号:A2015504)

摘　　要：目的:利用蛋白芯片技术检测神经梅毒患者和未累及神经系统的梅毒患者脑脊液中的差异蛋白,探索对神经梅毒具有诊断意义的细胞因子。方法:收集神经梅毒患者25例,未累及神经系统的梅毒患者21例,非感染性神经系统疾病患者14例,蛋白芯片检测脑脊液中的蛋白,ELISA确证差异蛋白的表达。结果:蛋白芯片分析发现相较于未累及神经系统的梅毒组,神经梅毒组脑脊液有41种蛋白显著升高,ELISA检测结果显示,神经梅毒组的脑脊液uPA浓度[(109.1±7.88)pg/m L]显著高于梅毒组uPA浓度[(63.86±4.53)pg/m L],差异有统计学意义(t=4.73,P<0.01),梅毒组与非感染性神经系统疾病组的脑脊液uPA浓度无显著差异;脑脊液uPA水平与脑脊液蛋白浓度(r=0.45,P=0.002)和VDRL滴度(r=0.49,P<0.01)呈显著正相关。结论:脑脊液uPA可能可作为神经梅毒新的诊断指标。Objective：To explore the new diagnosis biomarker of neurosyphilis through protein microarray. Methods： Cerebrospinal fluid （CSF） was obtained from 25 neurosyphilis patients, 21 syphilis patients without central nervous system involvement and 14 patients with non infectious nervous system disease. Protein microarray and ELISA were used to detect the protein expression of CSF. Results：Forty-one proteins were increased in the CSF of neurosyphilis patients compared with syphilis patients without neural involvement. ELISA confirmed that uPA levels were significantly higher in the CSF of neurosyphilis patients E （ 109. 1 ± 7.88 ） pg/mL versus （ 63.86± 4.53） pg/mL, t =4.73, P 〈0.01 1. There was also a clear correlation between CSF uPA levels and CSF-VDRLtiters （r=0.49, P〈0.01） and CSF protein levels （r =0.45, P=0.002）. Conclusion：The CSF uPA could be a new diagnosis biomarker of neurosyphilis.

关 键 词：神经梅毒 蛋白芯片 脑脊液 尿激酶型纤溶酶原激活剂 

分 类 号：R759.13[医药卫生—皮肤病学与性病学]