糖尿病神经病变患者单核细胞TOLL样受体4的表达及其与血浆肿瘤坏死因子α、白介素6的相关性研究  

Expression of TLR4 in monocytes and its correlation with TNF-α and IL-6 in patients with diabetic neuropathy

作　　者：孟庆胜[1] 朱涛[2] MENG Qing-sheng ZHU Tao(Department of Anesthesiology, Nanjing Hospital Affiliated to Nanjing Medical University ~ Nanjing First Hospital ,Nanjing 210029 ,China)

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)麻醉科,210029 [2]上海市交通大学附属第一人民医院松江分院麻醉科

出　　处：《中国糖尿病杂志》2017年第2期97-102,共6页Chinese Journal of Diabetes

基　　金：上海市科委医学引导类项目(134119b2700)

摘　　要：目的研究T2DM伴糖尿病神经病变患者外周血单核细胞TOLL样受体4(TLR4)的表达及其与促炎因子TNF-α、IL-6的相关性。方法将研究对象分为正常组(NC,n=44),T2DM组(n=44),T2DM神经病变组(n=44)。分离和提取外周血单核细胞,流式细胞术和RT-PCR检测单核细胞表面TLR4蛋白和mRNA;ELISA检测血浆TNF-α和IL-6。结果 T2DM神经病变组HbA_1 c[(9.09±-1.62)%vs(8.36土1.10)%vs(5.30±0.89)%]、单核细胞TLR4蛋白[(42.02±9.69)%vs(31.27±6.87)%vs(11.96±5.54)%]和TLR4 mRNA[(2.98±1.06)vs(1.74±0.47)vs(1.12±0.52)]、血浆TNF-α[(8.75±3.14)vs(6.27土3.64)vs(3.19±1.17)Pg/m1]、IL-6[(3.63±1.81)vs(2.60±1.14)vs(1.54±0.58)pg/ml]水平均高于T2DM和NC组(P<0.01);T2DM神经病变组TLR4蛋白与TNF-α、IL-6呈正相关(r=0.631,P<0.0001;r=0.447,P=0.0023)。结论糖尿病神经病变患者单核细胞TLR4参与机体炎症反应,与糖尿病神经病变的发生发展密切相关。Objective To investigate the expression of Toll-like receptor 4（TLR4） in peripheral blood monocytes and its correlation with TNF-α and IL-6 in type 2 diabetes（T2DM） patients with neuropathy.Methods Healthy volunteers（NC,n=44）,T2 DM without neuropathy（n=44） and T2 DM with neuropathy（n=44） were recruited in the present study.The expression of TLR4 mRNA and protein in peripheral blood monocytes was detected by RT-PCR and flow cytometry.ELISA was undertaken to measure the plasma concentration of TNF-α and IL-6.Results The levels of HbA1 c[（9.09± 1.62）%vs（8.36±1.10）%vs（5.30±0.89）%],the protein expression of TLR4[（42.02±9.69）%vs（31.27±6.87）%vs（11.96±5.54）%],the mRNA expression of TLR4[（2.98＋1.06）vs（1.74±0.47） vs（1.12±0.52）],TNF-α[（8.75±3.14）vs（6.27±3.64）t/s（3.19±1.17）pg/ml]and IL-6[（3.63±1.81）w（2.60±1.14）vs（1.54±0.58）pg/ml]in patients with diabetic neuropathy significantly increased compared with T2 DM and,NC group;TLR4 showed a positive correlation with TNF-α and IL-6（r=0.631,P〈0.0001;r=0.447,P=0.0023）.Conclusion TLR4 of monocytes may take part in the occurrence of systemic inflammation and has a close relationship with the development of diabetic neuropathy.

关 键 词：糖尿病神经病变 TOLL样受体4 外周血单核细胞 肿瘤坏死因子Α 白介素6 

分 类 号：R587.2[医药卫生—内分泌]