反相高效液相色谱法测定花生中的β-谷甾醇  

Determination of β-sitosterol in Peanut by Reversed-Phase High Performance Liquid Chromato Graphy

作　　者：张志舟 蔡大川[1] 郑家概[1] 张飞[1] 王和平[1] 许泽群[1] 丁少曼 陈轶[1] 蔡群娣 梁柳咏 Zhang Zhizhou Cai Dachuan Zheng Jiagai Zhang Fei Wang Heping Xu Zequn Ding Shaoman Chen Yi Cai Qundi Liang Liuyong(Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center（ Guangzhou）, Guangzhou 510070, China)

机构地区：[1]中国广州分析测试中心广东省化学危害应急检测技术重点实验室,广东广州510070

出　　处：《山东化工》2017年第5期80-82,共3页Shandong Chemical Industry

摘　　要：建立了测定花生中β-谷甾醇的反相高效液相色谱法。样品经过氢氧化钾—乙醇皂化提取后,采用高效液相色谱法进行含量测定。色谱条件为:C18柱(4.6 mm×250 mm,5μm);流动相为乙腈/异丙醇=50/50;流速1.0 mL/min;紫外检测器:波长210 nm;进样量10μL,外标法定量。结果表明:β-谷甾醇在5.0~250 mg/L范围内线性良好,线性相关系数为0.9999,方法回收率为90%~103%,RSD为3.74%,检出限为3.0 mg/kg。本方法准确、重复性好、灵敏度高,可用于花生中β-谷甾醇的含量测定。A new method for determination of β -sitosterol in peanut by reversed -phase high performance liquidchromatography. Peanuts were analyzed by high performance liquid chromatography after saponification with KOH - ethanolsolution. The separation of β- sitosterol was conducted on a Cls column （4.6 mm x 250 mm,Sμm ） with the mobile phase ofacetonitrile and isopropanol（50/50）. The flow rate was 1.0 mL/min and injection volume was 101xL. The detection wavelengthwas 210 nm and quantified by the external standard method. The linearity were obtained in the range of 5.0 - 250 mg/L withcorrelation coefficients 0.9999. The average recoveries of samples was in the range of 90% - 103% and RSD was 3.74%. Thedetermination of limit was 3.0mg/kg. The results indicate that the method is accurate ,reliable and reproducibility in the analysisof peanut.

关 键 词：Β-谷甾醇 花生 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]