野山参与园参rDNA-ITS序列比较分析  

Comparative Analysis of Wild Ginseng and Cultivated Ginseng in rDNA-ITS Sequence

作　　者：王晶[1] 付宝玉[1] 宋卓[1] 尹旭东[1] 孙立伟[1] 麻锐[1] Wang Jing Fu Baoyu Song Zhuo Yin Xudong Sun Liwei Ma Rui(College of Chemistry and Biology, Beihua University, Jilin 132013, China)

机构地区：[1]北华大学化学与生物学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2017年第2期182-185,共4页Journal of Beihua University（Natural Science）

基　　金：国家自然科学基金项目(81373932);吉林省大学生创新创业训练计划项目(201510201058);吉林省教育厅科学技术研究项目(201676);吉林省科技发展计划项目(20150520138JH)

摘　　要：目的比较野山参与园参rDNA-ITS序列差异,寻找其DNA分子鉴定方法.方法提取野山参与园参总DNA,扩增其ITS序列,运用Clustal X等软件比较分析野山参与园参ITS序列特征.结果野山参与园参经PCR扩增后分别获得700 bp和500 bp两条条带.其中对野山参和园参的700 bp条带测序分析后未发现差异位点,而二者的500 bp条带测序结果相似性仅为38%.结论该结果为野山参的鉴别提供了实验依据.Objective The difference of rDNA-ITS sequences between wild ginseng and cultivated ginseng was compared for building DNA molecular identification method.Method The total DNA of wild ginseng and cultivated ginseng was extracted.ITS sequences of both were amplified and analyzed by Clustal X software.Results The PCR results of wild ginseng and cultivated ginseng obtained two bands,700 bp and 500 bp respectively.There was no difference between wild ginseng and cultivated ginseng in the sequencing of 700 bp band and only 38% similarity in the sequencing of 500 bp band.Conclusion This results provided the experimental bases for the identification.

关 键 词：野山参 园参 rDNA-ITS序列 鉴别 质量评价 

分 类 号：R284.2[医药卫生—中药学]