松嫩盐碱草地旋覆花根围AM真菌侵染特性及多样性  

Infection characteristics and diversity of arbuscular mycorrhizal fungi in the rhizosphere of Inula japonica in Songnen saline-alkaline grassland

作　　者：杨春雪[1] 黄寿臣 陈飞[1] 李丽丽[1] Yang Chun-xue Huang Shou-chen Chen Fei Li Li-li(College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China)

机构地区：[1]东北林业大学园林学院,黑龙江哈尔滨150040

出　　处：《草业科学》2017年第2期231-239,共9页Pratacultural Science

基　　金：国家自然科学基金"星星草-丛枝菌根共生体应答盐碱逆境的生理机制解析(31601986)";中央高校基本科研业务费专项资金"松嫩盐碱草地星星草根围AM真菌多样性与功能研究(2572015CA22)";东北油田盐碱植被恢复与重建教育部重点实验室开放基金"松嫩盐碱草地马蔺根围AM真菌多样性研究(SAVER1608)"

摘　　要：为考察松嫩盐碱草地旋覆花(Inula japonica)根围AM真菌侵染特性及其多样性,本研究对黑龙江省肇东市西南部松嫩盐碱草地旋覆花根系和根际土壤进行分析。结果表明,在不同pH的土壤生境下,旋覆花根系均被AM真菌侵染,在土壤pH为8.76时,侵染率达到100%,泡囊丰度与丛枝丰度达到最大值,分别为75.67%和74.32%;在土壤pH为8.71时,侵染率为85%,泡囊丰度与丛枝丰度最低。通过形态学鉴定共分离出AM真菌8属37种,其中球囊霉属(Glomus)21种,占总种数的56.7%,为优势属,根内球囊霉(G.intraradices)和幼套球囊霉(G.etunicatum)是优势种;无梗囊霉属(Acaulospora)6种,占总种数的16.2%;盾巨孢囊霉属(Scutellospora)3种,占总种数的8.1%;和平囊霉属(Pacispora)、内养囊霉属(Entrophospora)各两种,各占总数的5.4%,巨孢囊霉属(Gigaspora)、多孢囊霉属(Diversispora)、原囊霉属(Archaeospora)各1种,各占总数的2.7%。In order to inspect the infection characteristic and diversity of arbuscular mycorrhizal（AM）fungi in the rhizosphere of Inula japonicain the Songnen saline-alkaline grassland in Zhaodong,Heilongjiang Province,roots and rhizosphere soil of I.japonica were collected from the southwest part of the grassland in July 2015.All roots,despite different soil pH levels,were infected by AM fungi.The infection rate reached 100% at pH8.76,when the abundance of arbuscules and vesicles also reached maximums of 75.67% and 74.32% respectively.When the soil pH was 8.71,the infection rate was 85%,and the abundance of arbuscules and vesicles were at their lowest levels.37 AM fungal species from 8genera were isolated by mor-phological analysis.The21 species belonging to Glomus accounted for 56.7% of all species,making Glomus the dominant genus;G.intraradices and G.etunicatum were the most common species.There were 6species of Acaulospora（16.2% of all species）,3species of Scutellospora（8.1% of all species）,2species of each Pacisporaand Entrophospora（each 5.4% of all species）,and 1species of each Gigaspora,Pacispora,and Entrophospora（each 2.7% of all species）.

关 键 词：松嫩盐碱草地 AM真菌 旋覆花 物种多样性 

分 类 号：S154.3[农业科学—土壤学] S682.19[农业科学—农业基础科学]