ATP生物荧光法检测结果与细菌含量相关性研究  

Study on correlation of bacterium contents and test result of ATP bioluminescence method

作　　者：许慧琼[1] 王一梅[1] 王斌[1] 梁建生[1] 

机构地区：[1]武汉市疾病预防控制中心,湖北武汉430015

出　　处：《中国消毒学杂志》2017年第2期140-144,147,共6页Chinese Journal of Disinfection

基　　金：武汉市卫生计生委2015年度医疗卫生科研项目(WG15D08)

摘　　要：目的研究ATP生物荧光法检测结果与试验菌菌落数的相关性,评价该方法的准确性和可靠性。方法分别用3种ATP生物荧光检测仪和菌落计数法检测金黄色葡萄球菌、大肠杆菌和白色念珠菌梯度稀释的菌悬液,构建数学模型,用残差图进行模型验证。分别用相关系数r和组内相关系数ICC评价准确性和可靠性。结果 3种检测仪检测试验菌在检测范围内均为直线模型较合适,3种试验菌的lg(菌落数)与3种检测仪lg(RLU)间的r均在0.9以上,BT-112D、hygiena System SURE Plus和Clean-Trace NGi的ICC分别为0.68、0.92和0.82。结论lg(RLU)与lg(菌落数)基本成直线正相关,ATP生物荧光法准确性较好,可靠性尚可,适合作为一种初筛、预警或现场教育方法应用于医疗机构、托幼机构、疫源地等物体表面、手卫生等清洁消毒效果监测。Objective To study the correlation between the results of ATP bioluminescence method and the colony forming units of testing microorganism,and evaluate the accuracy and reliability of ATP bioluminescence method. Methods Three kinds of ATP bioluminescence detectors and colony counting method were used to detected the Staphylococcus aureus,Escherichia coli and Candida albicans respectively. Build the mathematical model,and check the model by residual figure. Besides,linear correlation coefficient（ r） and intra- class correlation coefficient（ ICC） were used to evaluate the accuracy and reliability. Results The linear model was appropriate within the scope of the test,and the r between lg（ cfu） and lg（ RLU） of three kinds of testing microorganisms were all above 0. 9. The ICC of BT- 112 D,hygiena System SURE Plus and Clean- Trace NGi were 0. 68,0. 92,and 0. 82 respectively. Conclusion The lg（ cfu） and lg（ RLU） are in linear positive correlation basically. The accuracy and reliability of ATP bioluminescence method are good,so it is suitable for cleaning and disinfection effect monitoring of surface and hand in medical institutions,kindergartens,and infectious focus as a preliminary screening,early warning or field education method.

关 键 词：ATP生物荧光法 菌落数 相关系数 组内相关系数 

分 类 号：R187[医药卫生—流行病学]