Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development  

作　　者：HE Jin-gang CHENG Yu-dou GUAN Jun-feng GE Wen-ya ZHAO Zhe 

机构地区：[1]Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Science, Shijiazhuang 050051, P.R. China [2]Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, P.R. China

出　　处：《Journal of Integrative Agriculture》2017年第2期471-477,共7页农业科学学报（英文版）

基　　金：supported by the Specific Financial Funds of Hebei Province,China (494-0502-JSN-7FB3)

摘　　要：According to synthetic pathway of plant chlorogenic acid （CGA）, the expression patterns of genes encoding enzymes that are associated with CGA synthesis were studied in normally developed Xuehua pear fruit. The study demonstrated that CGA content in peel and flesh of Xuehua pear decreased as fruit development progressed, with a higher level in peel. The expression levels of PbPAL 1, PbPAL2, PbC3H, PbC4H, Pb4CL 1, Pb4CL2, Pb4CL6, PbHC T1 and PbHC T3 genes decreased in fruit, which was consistent with the pattern of variation in CGA content. That indicated that these genes might be key genes for influencing fruit CGA synthesis in Xuehua pear. However, Pb4CL7 gene expression profile is not consistent with variation of CGA content, hence, it may not be a key gene involved in CGA synthesis.According to synthetic pathway of plant chlorogenic acid （CGA）, the expression patterns of genes encoding enzymes that are associated with CGA synthesis were studied in normally developed Xuehua pear fruit. The study demonstrated that CGA content in peel and flesh of Xuehua pear decreased as fruit development progressed, with a higher level in peel. The expression levels of PbPAL 1, PbPAL2, PbC3H, PbC4H, Pb4CL 1, Pb4CL2, Pb4CL6, PbHC T1 and PbHC T3 genes decreased in fruit, which was consistent with the pattern of variation in CGA content. That indicated that these genes might be key genes for influencing fruit CGA synthesis in Xuehua pear. However, Pb4CL7 gene expression profile is not consistent with variation of CGA content, hence, it may not be a key gene involved in CGA synthesis.

关 键 词：cinnamate 4-hydroxylase gene hydroxy cinnamoyl CoA shikimate/quinic acid hydroxycinnamoyl transferasegene p-coumarate 3＇-hydroxylase gene 4-hydroxycinnamoyl-CoA ligase gene phenylalanine ammonia lyasegene Xuehua pear 

分 类 号：S661.2[农业科学—果树学]