β-榄香烯调控CDK8-P21通路逆转肺癌化疗耐药的机制研究  

Mechanisms of Elemene Reversing Lung Cancer Cells' Chemotherapy Drug Resistance by Regulating and Controlling CDK8-P21 Pathways

作　　者：韦巧玲[1] 张永军[2] 张爱琴[2] 包文龙[2] 史华[2] 姜志明[2] 顾丹琳[2] 

机构地区：[1]浙江省立同德医院,浙江杭州310012 [2]浙江省肿瘤医院,浙江杭州310022

出　　处：《中华中医药学刊》2017年第3期642-644,I0019,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(LY12H290006)

摘　　要：目的:在前期研究的基础上,进一步研究榄香烯调控CDK8-P21通路逆转肺癌化疗耐药的机制。方法:复制小鼠Lewis肺癌模型,造模成功后随机分为四组:模型组、香烯组、多柔比星组及榄+多组,分别予生理盐水、榄香烯、多柔比星及二者联合用药7 d,取瘤组织,荧光定量PCR及Western Blot检测瘤组织中CDK8及P21基因、蛋白表达情况。结果:与模型组比较,多柔比星组肿瘤组织中CDK8及P21基因、蛋白表达均增加(P<0.05)。与榄香烯组比较,榄+多组中P21基因、蛋白表达增加(P<0.05),多柔比星组明显增加(P<0.01)。而CDK8基因、蛋白表达无明显差别(P>0.05)。结论:化疗药物会诱导CDK8-P21通路的激活;榄香烯通过抑制P21基因、蛋白表达逆转移肺癌化疗耐药,而与CDK8-P21通路无关。Objectives： On the basis of the previous studies, to research the mechanisms of Elemene reversing lung cancer cells＇ chemotherapy drug resistance by regulating and controlling CDK8 -P21 pathways. Methods： The mice model with Lewis lung cancer was duplicated. The mice were treated with Elemene, Doxorubicin and both together, respectively. After seven days, the gene and protein expression of CDK8 and P21 were detected with Realtime Fluorescence Quantitative PCR and Western Blot. Results： The P21 gene and protein expressions were increased in Doxorubicin group than those in model group as well as CDK8 gene and protein expressions （P 〈 0. 05 ）. Compared with those in Elemene group, the P21 gene and protein expressions were increased （P 〈 0.05 ） in Elemene ＋ Doxorubicin group, and obviously increased in Doxorubicin group （ P 〈 0. 01 ）. The CDK8 gene and protein expressions had no difference between Elemene group and other groups （P 〉 0. 05）. Conclusions： Chemotherapy drugs can activate CDK8 -P21 pathway. The Elemene reversed lung cancer cell resisting chemotherapy drug by inhibiting P21 gene and protein expression and had no relation- ship with CDK8 -P21 pathways.

关 键 词：CDK8 多药耐药 榄香烯 P21 

分 类 号：R285.5[医药卫生—中药学]