升陷汤对实验性自身免疫性重症肌无力大鼠免疫机制研究  

Immune Mechanism Research of Shengxian Decoction on Rats with Experimental Autoimmune Myasthenia Gravis

作　　者：朱洁[1] 程杨[1] 许骏尧 吴周烨 陈以狄 陈刚[1] 吴颢昕[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210023

出　　处：《中华中医药学刊》2017年第3期717-720,I0026,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省大学生科技创新训练计划省级重点项目(201410315003Z)

摘　　要：目的:探讨升陷汤治疗实验性自身免疫性重症肌无力(Experimental Autoimmune Myasthenia Gravis,EAMG)大鼠的免疫机制。方法:采用人工合成鼠源乙酰胆碱受体(acetylcholinergic receptor,ACh R)α亚基97-116肽段免疫Lewis大鼠制备EAMG模型,经模型评估确定建模成功后将大鼠随机分为佐剂对照组,模型对照组,西药对照组(强的松),升陷汤低、中、高剂量组。给药1个月后检测各组大鼠肌电图,血清中乙酰胆碱抗体(ACh R-Ab)滴度和IFN-γ(Interferon,IFN)含量,及外周血中淋巴细胞亚型CD_4^+、CD_8^+细胞及CD_4^+/CD_8^+的比例。结果:与佐剂对照组相比,造模组AChR-Ab、IFN-γ均显著上升(P<0.05);与模型对照组相比,给药组的AChR-Ab、IFN-γ含量都显著下降(P<0.05)。与模型对照组相比:升陷汤低、中剂量组CD_4^+/CD_8^+显著下降(P<0.01);西药对照组CD_4^+、CD_8^+、CD_4^+/CD_8^+显著下降(P<0.01)。结论:升陷汤可能通过升高CD_4^+淋巴细胞含量,降低CD_4^+/CD_8^+的比例,从而降低IFN-γ含量,进而抑制ACh Rab的合成或促进其降解,起到治疗EAMG大鼠的作用。Objective ： To explore the immune mechanism of Shengxian Decoction treating rats with experimental autoimmune myasthenia gravis （EAMG）. Methods：Lewis rats were made into the model of EAMG by injecting the artificially synthesized peptide derived from the rats＇ sequence 97 - 116 of the AChR a subunit （R97 - 116 ） , and further divided into the adjuvant control group,the model control group,western medicine control group（Prednisone） , Shengxian Decoction low, middle and high dose groups after successful model evaluation. And then the electromyography, acetylcholine receptor antibody（AChR - Ab） titer, IFN - γ content, the levels of CD4 and CDs lymphocyte in peripheral blood and CD4/CDs proportion in rats after receiving one - month treatment were tested and calculated. Results： Compared with the adjuvant control group,the levels of AChR- Ab and IFN-γ in the model groups both went up evidently（ P 〈 0. 05 ） while compared with the model control group, the levels of AChR - Ab and IFN - γ in the treatment groups both declined notably （P 〈 0. 05）. Compared with the adjuvant control group, the CD4/CDs proportion in the model groups went up evidently（ P 〈0. 01 ） and the proportion among Shengxian Decoction low and middle dose groups tended to decline. CD4 and CD8 levels and CD4/CDs proportion in the western medicine control group all went down evidently（ P 〈 0. 01 ）. Conclusion ： Shengxian Decoction can inhibit the synthesis of AChR - Ab or promote its degradation by decreasing CD4＋ / CDs proportion and IFN - γ content so as to reach the function of treating rats with EAMG.

关 键 词：实验性自身免疫性重症肌无力 升陷汤 ACHR-AB IFN-Γ 淋巴细胞亚群 

分 类 号：R285.5[医药卫生—中药学]