对乙基多杀菌素中度抗性降低黄胸蓟马的适合度  

Moderate resistance to spinetoram reduces the fitness of Thrips hawaiiensis( Thysanoptera: Thripidae)

作　　者：付步礼[1] 李强[1,2] 夏西亚 唐良德[1] 邱海燕[1] 谢艺贤[1] 曾东强[2] 刘奎[1,4] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海口571101 [2]广西大学农药与环境毒理研究所,南宁530005 [3]华中农业大学植物科学技术学院,武汉430070 [4]农业部热带作物有害生物综合治理重点实验室,海口571101

出　　处：《昆虫学报》2017年第2期180-188,共9页Acta Entomologica Sinica

基　　金：中央级科研院所基本业务费项目(2016hzs1J007);海南省自然科学基金项目(20153066);现代农业产业技术体系建设专项资(CARS-32-04)

摘　　要：【目的】评估比较黄胸蓟马Thrips hawaiiensis(Morgan)乙基多杀菌素中等抗性种群与敏感种群的生物学特性,进一步明确黄胸蓟马对乙基多杀菌素的抗性规律及抗性风险,以阐明该虫的灾变规律并为其科学防治提供理论依据。【方法】采用群体淘汰法获得黄胸蓟马乙基多杀菌素中抗种群,并与实验室敏感种群比较,观察记录其种群生物学特征;组建抗性、敏感种群生命表,并计算种群相对适合度(Rf)。【结果】与敏感种群比较,黄胸蓟马中度抗乙基多杀菌素种群的幼期各历期均有不同程度延长,雌雄成虫寿命均显著缩短,且繁殖力明显降低。敏感种群的内禀增长率(r=0.1753)和周限增长率(λ=1.1916)均高于抗性种群(r=0.1554,λ=1.1681),表明乙基多杀菌素产生中等抗药性降低了黄胸蓟马种群的适合度,使得该种群增殖能力减弱。中抗乙基多杀菌素种群的净生殖率(R_0)(44.12)低于敏感种群的R0(56.96)。中度抗乙基多杀菌素种群的平均世代周期(T=24.37 d)显著长于敏感种群(T=23.06 d)。以两种群的内禀增长率(r)计算得到的中度抗性种群的相对适合度(Rf)=0.886。【结论】本研究明确了黄胸蓟马中度抗乙基多杀菌素种群繁殖力、种群增长受到抑制,具有生存劣势;但未来仍需长期追踪该抗性的演化。本研究同时指出以内禀增长率计算相对适合度的合理性。此外,本研究将存活率进行Weibull分布拟合,虽然决定系数很高,但拟合曲线仍有许多待商榷之处,需谨慎使用。【Aim 】This study aims to compare the differences in demographic characteristics between susceptible and moderate spinetoram-resistant populations of Thrips hawaiiensis（ Morgan）,and to make clear the resistance regularity and resistance risk of this insect to spinetoram,so as to clarify its disaster rule and to provide theoretical basis for its scientific control. 【Methods】The resistant population was developed by spinetoram selection,and compared with the susceptible population by using the age-stage,two-sex life table. Moreover,the relative fitness（ Rf） was calculated based on the intrinsic rate ofincrease. 【Results 】 Compared with the susceptible population, the moderate spinetoram-resistant population had a longer duration of immature stages,shorter adult longevity and lower fecundity. The net reproductive rate R0,intrinsic rate of increase r,finite rate of increase λ,and mean generation time T for the spinetoram-resistant population were 44. 12 offsprings,0. 1554 d^-1,1. 1681 d^-1,and 24. 37 d,respectively,while those for the susceptible population were 56. 96 offsprings,0. 1753 d^-1,1. 1916 d^-1,and 23. 06 d,respectively. Based on the R0 values,the relative fitness of the spinetoram-resistant population was 0. 886. 【Conclusion】This study revealed the biological fitness costs in T. hawaiiensis population with moderate resistance to spinetoram. It is suggested that the calculation of relative fitness should be based on the intrinsic rate of increase. Moreover,fitting the survival curve to Weibull distribution should be carried out with caution.

关 键 词：黄胸蓟马 乙基多杀菌素 抗药性 生物学 适合度代价 

分 类 号：Q965.9[生物学—昆虫学]