β-catenin对人牙周膜干细胞应力成骨调控作用的研究  

Study on the β-catenin regulating osteogenesis of human periodontal ligament stem cells under mechanical tension

作　　者：秦凡[1] 周述作 罗金英[1] 寇婷婷[1] 崔岳[1] 蔡江文 周继祥[1] QIN Fan ZHOU Shu-zuo LUO Jin-ying KOU Ting-ting CUI Yue CAI Jiang-wen ZHOU Ji-xiang(Department of Stomatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, Chin)

机构地区：[1]第三军医大学西南医院口腔科,重庆400038

出　　处：《局解手术学杂志》2017年第3期162-166,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(30870599)

摘　　要：目的探讨β-catenin对人牙周膜干细胞应力下成骨的影响。方法体外分离培养人牙周膜干细胞,以β-catenin为靶点的激动剂和抑制剂上调或下调其β-catenin蛋白的表达,用Western blot检测不同时段(0、6、12、24 h)的应力作用下,激动剂或抑制剂处理后PDLSCs中β-catenin的表达水平及相关成骨标志物ALP、BMP2、Runx2的表达。结果在外源性张应力作用下,成骨相关标志物ALP、Runx2较无张力组的表达水平增加(P<0.05);β-catenin激动剂WAY-262611作用后的PDLSCs,在外源性张应力条件下,成骨相关标物ALP的表达在加力0~12 h时段较DMSO加力组(对照组)明显增强(P<0.05)。结论 Wnt/β-catenin信号通路参与了PDLSCs早期的应力条件下成骨。Objective To investigate the effect of β-catenin on the osteogenic differentiation of human periodontal ligament stem cells （PDLSCs） under mechanical tension. Methods PDLSCs were seperately euhured in vitro from the periodontium attached at freshly extracted teeth. β-catenin-targeting agonist or inhibitor was applied to the PDLSCs to upregulate or downregulate the expression of activity of β-catenin protein. The expression of β-catenin protein and the osteogenetic related markers （ALP, BMP2, Runx 2） under the mechanical tension with different period（0,6,12,24 h） were assessed with Western blot in the PDLSCs irritated with agonist or inhibitor. Results Compared with the PDLSCs without mechanical tension, the expression of osteogenesis related marker proteins, ALP and Runx 2, in PDLSCs were enhanced under the circular mechanial tensile stress （ P 〈 0.05 ）. In the PDLSCs irritated with WAY-262611, the 13-catenin-targeting agonist, the expression of osteogenetic related markers ALP in the PDLSCs was improved during the first 12 h period of mechnical tensile load, which was significantly higher than that in the DMSO group. Conclusion Wnt/β-catenin signaling pathway plays an important role in the early osteogenesis differentiation of PDLSCs under cyclic tension. The β-catenin promotes osteogenic differentiation of PDLSCs under mechanical tension.

关 键 词：人牙周膜干细胞 WNT/Β-CATENIN信号通路 成骨分化 外源性张应力 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]