乌头叶面微生物菌群变化特征及其与乌头霜霉病相关性  

Variation Characteristics of Phylloplane Microbies from Aconitum carmichaeli and Correlation with Downy Mildew

作　　者：李娜[1] 胡亮[1] 王婷[1] 欧洪[1] 王光志[1] 

机构地区：[1]成都中医药大学,中药材标准化教育部重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2017年第5期42-46,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30901962);四川省教育厅重点项目(15ZA0098)

摘　　要：目的:以四川江油栽培乌头为研究对象,探索乌头叶面微生物菌群动态变化特征及其与乌头霜霉病的相关性。方法:收集不同病害等级的乌头霜霉病叶片,摇床震荡与超声结合的方法提取乌头叶面微生物,稀释平板计数法测定乌头叶面细菌,真菌数量。结果:乌头霜霉病发病情况与环境温度相关,患病乌头叶面细菌,真菌数量相较于健康乌头都有所增加,且叶面其他真菌数量随着病害程度的逐渐加重而呈现出较有规律的逐渐增加趋势。结论:乌头霜霉病在其生长期的3月末至5月末发病迅速,且日平均气温15~20℃比较适宜乌头霜霉病病原菌的生长繁殖。乌头叶面真菌数量的变化与乌头霜霉病病害程度呈正相关。Objective： To explore the dynamic change characteristics of phylloplane microbe from Aconitum carmichcteli and its correlation with Aconitum downy mildew, with those cultivated in Jiangyou Sichuan as the research subjects. Method： The leaves with downy mildew disease at different levels were collected. Foliar microbes were extracted by combined shock shaker and ultrasound method. The count of bacteria and fungi were measured by dilution plate count method. Result： Aconitum downy mildew was associated with environmental temperature. The count of phylloplane bacteria and fungi in the leaves suffering from downy mildew of A. carmichaeli was increased as compared to healthy one, and the count of other fungi showed a more regular upward trend with the increasing degree of downy mildew disease of A. carmichaeli. Conclusion： Aconitum downy mildew from A. carmichaeli had rapid onset in its growth period at the end of March to the end of May, and the daily average temperature between 15 ℃ to 20 ℃ was more appropriate for the growth and reproduction of Aconitum downy mildew pathogens. Changes in count of phylloplane fungi from A. carmichaeli were positively correlated with the degree of Aconitum downy mildew disease.

关 键 词：乌头 霜霉病 叶面 细菌 真菌 

分 类 号：R282[医药卫生—中药学]