甘草对芫花抗小鼠肝癌腹水作用的影响  

Effect of Combination of Glycyrrhizae Radix et Rhizoma and Genkwa Flos Air in Resisting Hepatocellular Carcinoma Ascites in Mice

作　　者：李玉婷[1,2] 闫晨[2] 郭晓东[3] 郭秋岩 杨美[3] 张彦琼 林娜[1,2] 

机构地区：[1]承德医学院,河北承德067000 [2]中国中医科学院中药研究所,北京100700 [3]解放军第302医院病理诊断与研究中心,北京100039

出　　处：《中国实验方剂学杂志》2017年第5期107-112,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB505300;2011CB505305)

摘　　要：目的:观察不同配比甘草-芫花煎剂对肝癌腹水模型小鼠的干预影响,并探讨其作用机制。方法:取昆明种雄性小鼠135只,随机分为正常组、模型组、芫花单煎组、芫花-甘草(1∶0.25,1∶0.5,1∶1,1∶2,1∶4)组和甘草单煎组。除正常组外,其他组小鼠腹腔均接种肝癌腹水瘤细胞系H22,并分别灌胃给药,正常组和模型组给予等容积生理盐水。12 d后分别测量各组小鼠的腹水量、腹围和体重及肝肾指数,蛋白质免疫印迹(Western blot)法测定肾脏组织中水通道蛋白2(AQP2)和肾加压素受体(V2R)的蛋白表达,生化法测定小鼠血清中丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),尿素氮(BUN)和尿肌酐(Cr)的水平。结果:与正常组比较,模型组小鼠的腹水量、腹围、体重、肝指数及血清中ALT,AST,BUN,Cr水平均显著升高(P<0.01),肾指数显著降低(P<0.01),AQP2,V2R蛋白的表达显著升高(P<0.01)。与模型组比较,芫花单煎组和芫花-甘草1∶0.5,1∶1和1∶2组均能显著降低小鼠的腹水量和体重(P<0.05,P<0.01);芫花单煎组和芫花-甘草1∶1组同时能显著降低小鼠的腹围(P<0.05,P<0.01),降低AQP2,V2R蛋白的表达(P<0.05,P<0.01)。与芫花单煎组比较,芫花-甘草1∶1组能显著降低小鼠的腹水量、腹围和体重(P<0.05,P<0.01),降低AQP2,V2R的表达(P<0.05),芫花-甘草1∶4组则使其显著升高(P<0.05,P<0.01)。结论:不同比例甘草的配伍能增强或抵消芫花抗小鼠肝癌腹水的作用,且相关变化可能与其有效调节了肾组织内的AQP2和V2R蛋白表达有关。Objective： To investigate the effect of combination of Glycyrrhizae Radix et Rhizoma and Genkwa Flos with different proportions in resisting hepatocellular carcinoma （HCC） ascites in mice. Method： A total of 135 male Kunming mice were randomly divided into normal control group, model group, Genkwa Flos and Glycyrrhizae Radix et Rhizoma combination groups （ ratios of 1 ： 0.25, 1 ： 0.5, 1 ： 1, 1 ： 2, 1 ： 4） , pure Genkwa Flos group and pure Glycyrrhizae Radix et Rhizoma group. HCC ascites model was established by inoculating H22 cells intraperitoneally in all groups except for the normal group. HCC ascites mice in drug-treatment groups were intragastrically administrated with the corresponding drugs, while the normal and model groups were given the equal volume of normal saline. At 12 d, ascites amount, abdominal girth, weight, and liver and kidney indicators were measured respectively. Expressions of aquaporin-2 （AQP2） and renal vasopressin receptors-2 （V2R） in the kidney tissues were detected by Western blot. Alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, blood urea nitrogen （BUN） and creatinine （Cr） were determined by the biochemistry analyzer. Result： The model group showed significant higher weight, abdominal girth, ascites, liver index, ALT, AST, BUN, Cr and lower kidney index than normal control group （P 〈0.01 ） , with notable rise in AQP2 and V2R protein expressions. Compared with the normal group, the pure Genkwa Flos group and Genkwa Flos and Glycyrrhizae Radix et Rhizoma groups showed significant reduction in weight, abdominal girth, ascites and expressions of aquaporin-2 and renal vasopressin receptors-2 in the kidney tissues （P 〈 0.05）. Genkwa Flos and Glycyrrhizae Radix et Rhizoma group （ 1 ： 1 ） also showed dramatic increases in weight, abdominal girth, ascites and expressions of aquaporin-2 and renal vasopressin receptors-2 in the kidney tissues （P 〈 0.05 ）. Conclusion： The combination of Genkwa Flos and Gly

关 键 词：芫花 甘草 十八反 肝癌腹水 水通道蛋白2 肾加压素受体 

分 类 号：R285.5[医药卫生—中药学]