L-NAME诱导妊娠高血压大鼠动脉重建的特征  

Characteristics of vascular remodeling in pregnant hypertensive rats induced by L-NAME

作　　者：刘妍妍[1] 姚庆苹[1] 沈宝荣[1] 齐颖新[1] 张萍[1] 高立志[1] 

机构地区：[1]上海交通大学生命科学技术学院力学生物学研究所,上海200240

出　　处：《医用生物力学》2017年第1期66-71,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(11102112;11372190)

摘　　要：目的探讨妊娠高血压大鼠动脉重建的特征。方法采用亚硝基左旋精氨酸甲酯(L-nitro-arginine methylester,L-NAME)诱导,构建妊娠高血压大鼠模型,以正常孕鼠为对照,用无创鼠尾血压测量仪测量血压以确认模型构建成功。测量血液黏度、左侧颈总动脉平均血流量,检测胸主动脉和颈总动脉的管壁厚度、管腔内径、张开角,计算并比较颈总动脉平均切应力的变化。Western blotting检测胸主动脉血管成分胶原蛋白Ⅰ(collagenⅠ,ColⅠ)、胶原蛋白Ⅲ(collagenⅢ,ColⅢ)表达,分析动脉血管重建程度。结果妊娠高血压模型大鼠血黏度增加,平均血流量降低;颈总动脉和胸主动脉壁厚内径比明显增大;颈总动脉张开角减小,胸主动脉张开角增大;颈总动脉平均切应力降低(28.52±3.08)%;ColⅠ表达下降,ColⅢ表达增加,ColⅠ、Ⅲ比例显著减小。结论妊娠高血压大鼠平均切应力下降,胸主动脉和颈总动脉发生血管重建。研究结果可以为妊娠高血压发病机制的深入研究提供基础实验数据支撑。Objective To elucidate the characteristics of vascular remodeling in pregnant hypertensive rats. Meth- ods Pregnant rats were induced by L-nitro-arginine methylester （L-NAME） to build hypertension models and normal pregnant rats were used as control. Using a programmable sphygmomanometer, the blood pressure was recorded with the tail-cuff method to ensure the hypertension model was successfully replicated. The changes of mean shear stress were determined after the blood viscosity, the average blood flow and the inner diameter in left common carotid artery were measured. To analyze the degree of arterial remodeling, the protein expression levels of collagen Ⅰ （Col Ⅰ） and Ⅲ （Col Ⅲ） were detected by Western blotting, and the media thickness, the inner diameter, the opening angel both in thoracic aorta and carotid artery were determined. Results The mean shear stress of common carotid artery was reduced by （28.52 ± 3.08） % with the blood viscosity increasing and the average blood flow decreasing in pregnant hypertensive rats. Compared with control groups, the ratio of media thickness and inner diameter significantly increased in thoracic aorta and carotid artery, while the opening angel decreased in carotid artery and increased in thoracic aorta. With the expression of Col Ⅰ decreasing and Col Ⅲ increasing, the ratio of Col Ⅰ and Col Ⅲ went an apparent decline. Conclusions The mean shear stress is descending in pregnant hypertensive rat, with the remodeling of thoracic aorta and carotid artery. These results may provide new experimental references for further illustrating pathogenesis of pregnant hypertension.

关 键 词：妊娠高血压 大鼠模型 血管重建 切应力 

分 类 号：R318.01[医药卫生—生物医学工程]