牦牛隐性乳房炎病原菌的分离及鉴定  

Isolation and Identification of Yak Subclinical Mastitis Pathogenic Bacteria from Milk Samples

作　　者：李小旭[1] 刘侃[1] 张小琴 郝葆青[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]阿坝师范学院化学化工与生命科学系,四川汶川623002

出　　处：《四川畜牧兽医》2017年第3期36-38,共3页Sichuan Animal & Veterinary Sciences

摘　　要：牦牛隐性乳房炎被认为是高原藏族地区牦牛奶产量低下的主要原因之一。探索和研究牦牛隐性乳房炎致病菌特性及流行病学特征,可为牦牛隐性乳房炎防治提供一定的依据。本试验采集了红原地区不同牧群牦牛的80份奶样,由体细胞数、p H值测定初步筛选出了30份疑似牦牛乳房炎奶样,然后对其进行培养观察及生化检测,分离并鉴定出了牦牛隐性乳房炎病原菌。结果显示:80份奶样中有28份为牦牛隐性乳房炎奶样,检出率为35%;细菌学检测表明,28份奶样中金黄色葡萄球菌占10.71%,无乳链球菌占25%,乳房链球菌占32.14%,停乳链球菌占17.86%,大肠杆菌占14.29%。Yak subclinical mastitis is considered one of the primary causes for decreasing the milk yield of yak in Tibetan plateauo It has great practical significance that explore and research the characteristic of pathogenic bacterium and epidemiology characteristic, could provide certain basis for the prevention of yak subclinical mastitis at Hongyuan Tibetan area. In this study, 80 milk samples were collected from three different herd in Hongyuan county, 30 milk samples were screened as suspected yak subclinical mastitis by milk somatic cell count and pH value. Afterwards, the suspected milk samples were cultivated for observation, biochemical identification, and other further testing. The results showed that, 28 milk samples were subclinical mastitis in the 80 milk samples, its detection rate was 35%. Bacteriological tests indicated that among the 80 milk samples, staphylococcus aureus was aceount for 10.71%, streptococcus agalactiae was account for 25%, streptococcus uberis was account for 32.14%, streptococcus dysgalactiae was account for 17.86%, Escherichia coli was account for 14.29%.

关 键 词：牦牛 隐性乳房炎 病原菌 奶样 分离 鉴定 

分 类 号：S858.23[农业科学—临床兽医学]