1型糖尿病并发慢性胆囊炎患者外周血IL-6基因多态性研究  

Peripheral blood IL-6 gene polymorphism in patients with type 1 diabetes mellitus complicated by chronic cholecystitis

作　　者：吴军[1] 尚瑞[1] Wu Jun Shang Rui.(Department of Gastroenterology,Affiliated Hospital,Hubei Medical and Pharmaceutical College, Shiyan 442000,Hubei Provinc)

机构地区：[1]湖北医药学院附属人民医院消化内科,湖北省十堰市442000

出　　处：《实用肝脏病杂志》2017年第2期207-210,共4页Journal of Practical Hepatology

摘　　要：目的分析外周血IL-6 174C/G基因型和等位基因频率在研究人群中的分布情况,以探讨1型糖尿病(T1DM)并发慢性胆囊炎患者外周血IL-6基因多态性的变化。方法收集1型糖尿病并发慢性胆囊炎患者110例和健康对照者120例。采用聚合酶链反应-限制性片段长度多态性法对所有受检者进行外周血基因多态性检测,计算基因型频率和等位基因频率,并结合问卷调查和临床生化检查的相关信息进行病例对照分析研究。应用SPSS 17.0统计软件包行统计分析。结果纳入本研究的两组人群性别和年龄具有可比性,1型糖尿病并发慢性胆囊炎患者体质指数(BMI)为(28.15±3.98)kg/m^2,显著高于健康人的(26.86±6.52)kg/m^2(P<0.05);在110例1型糖尿病并发慢性胆囊炎患者中,伴有高血压现患例数65例(59.09%),显著高于健康人的32.50%(39/120,P<0.01);1型糖尿病并发慢性胆囊炎患者存在糖尿病家族史的比例显著高于健康人(54.55%对10.83%,P<0.01);1型糖尿病并发慢性胆囊炎患者吸烟(13.64%)和饮酒(19.09%)史的比例显著高于健康人(分别为11.67%和8.33%,P<0.01);1型糖尿病并发慢性胆囊炎患者外周血IL-6 174C/G基因CC基因型为40.91%,显著高于健康人的28.33%(P<0.05),而GG基因型为7.27%,显著低于健康人的19.17%(P<0.05),C等位基因分布为52.73%,显著高于健康人的15.00%(P<0.01);单因素分析结果提示,携带IL-6 174 C等位基因【(OR=1.357(1.037~1.776),P<0.05】、存在高血压现病【OR=1.324(1.187~1.476),P<0.01】、肥胖【OR=1.165(1.087~1.249)和有饮酒史【OR=1.113(1.046~1.184),P<0.01】为T1DM患者并发胆囊炎的危险因素;多因素Logistic回归分析结果提示携带IL-6 174C等位基因【OR值及其95%可信区间分别为1.258(1.119~1.413)】、高血压【1.225(1.032~1.454)】和肥胖【1.167(1.056~1.289),P<0.01】以及饮酒史【OR值为1.111(1.006~1.228),P<0.05】为T1DM患者并发胆囊炎的独立危险因素。结论 IL-6-174C/G基因多态性与1型糖Objective To analyzed the peripheral blood IL-6 174C / G genotype and allele frequencies for the investigation of the peripheral blood IL-6 gene polymorphism in patients with type 1 diabetes mellitus （T1DM） complicated by chronic cholecystitis. Methods 110 patients with T1DM complicated by chronic cholecystitis and 120 healthy individuals were included in this study. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was applied to the genetic polymorphism detection,and the genotype and allele frequencies were calculated. The clinical data and blood biochemistry index were obtained by questionnaires and routine detection. SPSS 17.0 statistical package was used for statistical analysis. Results The age and gender in the two groups were comparable,and the body mass index （BMI） in patients with T1DM complicated by chronic cholecystitis was （28.15±3.98） kg/m^2,much higher than （26.86±6.52） kg/m^2 in the controls（P〈0.05）;the prevalence of blood hypertension in patients with T1DM complicated by chronic cholecystitis was 59.09%,much higher than 32.50% in the control group （P〈0.01）;family history of diabetes in the former accounted for 54.55%,much higher than 10.83% in the controls （P〈0.01）;the smoking （13.64%） and alcohol consumption（19.09%） in patients with T1DM complicated by chronic cholecystitis were much higher than 11.67%and 8.33%（P〈0.01） in the control;the peripheral blood IL-6 174C/G gene CC genotype in patients was 40.91%,much higher than 28.33%（P〈0.05）,while GG genotype was 7.27%,much lower than 19.17% in the control （P〈0.05） and C allele frequencies was 52.73%,much higher than 15.00% in the control （P〈0.01）;the univariate analysis showed that carrying IL-6 174 C allele [（OR=1.357 （1.037N1.776）,P〈0.05], hypertension [ OR = 1.324 （ 1.187 - 1.476 ）, P〈0.01 ], obesity [ OR = 1.165 （ 1.087 N 1.249 ） and alcohol consumption [OR = 1.113 （1.046±1.184）,P〈0.01] were the risk factors for patients w

关 键 词：慢性胆囊炎 1型糖尿病 白细胞介素-6 基因多态性 

分 类 号：R587.2[医药卫生—内分泌] R575.61[医药卫生—内科学]