二氢杨梅素对小鼠急性脑缺血缺氧的保护作用  

Protective effect of dihydromyricetin on acute cerebral hypoxia-ischemia in mice

作　　者：韦坤璇[1] 邓之婧 甘露[2] 庞逸敏[2] 何萍[2] 郭哲[3] Wei Kunxuan Deng Zhijing Gan Lu Pang Yiming He Ping Guo Zhe(Department of Pharmacy, The First Affiliated Hospital of Guangxf Medical University, Nanning 530021,China Laboratory Ani- mal Center of Guangxi Medical University, Nanning 530021, China The 3th Affiliated Hospital of Guangxi Medical University, Nanning 530031, China)

机构地区：[1]广西医科大学第一附属医院药学部,南宁530021 [2]广西医科大学实验动物中心,南宁530021 [3]广西医科大学第三附属医院,南宁530031

出　　处：《广西医科大学学报》2017年第3期347-350,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81360056);广西自然科学基金资助项目(No.2012GXNSFCA053005;No.2013GXNSFAA019147);南宁市科学研究与技术开发计划项目(No.20153105)

摘　　要：目的:研究二氢杨梅素(DMY)对小鼠急性脑缺血缺氧的保护作用。方法:将雄性昆明小鼠随机分为空白对照组(或假手术组)、DMY高剂量组、DMY低剂量组和金纳多组,必要时设模型组,每组30只。DMY高、低剂量组分别灌胃DMY500mg/kg、250mg/kg,1次/d,共10d。末次给药1h后,采用小鼠断头实验,观察DMY对断头后小鼠喘气时间和喘气次数的影响;小鼠常压耐缺氧实验,观察DMY对小鼠存活时间、耗氧量和耗氧率的影响;小鼠结扎双侧颈总动脉实验,观察DMY对小鼠存活时间的影响。结果:与空白对照组或模型组比较,DMY高、低剂量可明显延长小鼠断头后喘气时间和喘气次数,降低常压缺氧小鼠耗氧率并延长双侧颈总动脉结扎小鼠的存活时间(P<0.05或P<0.01);此外,DMY高剂量还可延长常压缺氧小鼠存活时间(P<0.05)。结论:DMY对小鼠急性脑缺血缺氧具有一定的保护作用。Objective：To study the protective effect of dihydromyricetin （DMY） on acute cerebral hypoxia- ischemia in mice. Methods. Male Kuming mice were randomly divided into control or sham group, high- and low-dose DMY group, ginaton group and model group. The mice in the high- and low-dose DMY group were administered 500 mg/kg and 250 mg/kg DMY respectively via intragastric gavage for 10 days. At 1 h after the last administration, the gasping time and gasping number of beheaded mice, survival time, oxy- gen consumption ratio in hypoxia tolerance mice, and survival time in bilateral carotid artery ligation （BCAO） mice were observed. Results： High- and low-dose DMY could significantly increased the gasping time and gasping number of beheaded mice, prolonged the oxygen consumption ratio of hypoxia tolerance mice and the survival time of BCAO mice. High-dos of DMY could also prolong the surviving time of hy- poxia tolerance mice. Conclusion. DMY exerted a protective effect on acute cerebral hypoxia-ischemia in mice.

关 键 词：二氢杨梅素 急性脑缺血缺氧 小鼠 

分 类 号：R285.5[医药卫生—中药学]